CRM1-Mediated Nuclear Export Is Present During Porcine Embryogenesis, but Is Not Required for Early Cleavage

Regulated movement of cellular factors between the cytoplasm and nucleus is required for fundamental cellular processes ranging from cell cycle control to transcriptional regulation. CRM1 is a nuclear export factor whose function is to actively transport nuclear cargos that bear nuclear export seque...

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Bibliographic Details
Published in:Biology of reproduction 2002-09, Vol.67 (3), p.814-819
Main Authors: CABOT, Ryan A, HANNINK, Mark, PRATHER, Randall S
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cleavage Stage, Ovum
Culture Techniques
Early stages. Segmentation. Gastrulation. Neurulation
Embryo, Mammalian - chemistry
Embryology: invertebrates and vertebrates. Teratology
Embryonic and Fetal Development
Embryonic Development
Exportin 1 Protein
Fatty Acids, Unsaturated - pharmacology
Female
Fundamental and applied biological sciences. Psychology
Karyopherins - analysis
Karyopherins - antagonists & inhibitors
Karyopherins - physiology
Pregnancy
Receptors, Cytoplasmic and Nuclear
Swine - embryology
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Summary:Regulated movement of cellular factors between the cytoplasm and nucleus is required for fundamental cellular processes ranging from cell cycle control to transcriptional regulation. CRM1 is a nuclear export factor whose function is to actively transport nuclear cargos that bear nuclear export sequences to the cytoplasm. Because CRM1 likely plays a role in the intracellular regulation of many cellular processes, we set out to characterize CRM1 function during early mammalian embryogenesis. A series of embryo culture experiments that employed a specific inhibitor of CRM1, leptomycin B, indicated that CRM1 function is not required for development until after the 4-cell stage of porcine embryo development. Immunolocalization of CRM1 in fixed embryos revealed that CRM1 is localized in a unique pattern during the period of time when the embryo does not have a developmental requirement for CRM1. Despite these findings, a microinjection assay showed that CRM1 function persists during this period of development. This demonstrates that although CRM1 is present in a functional form throughout mammalian embryo development, its function is not required for early cleavage.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod.102.004960