Nucleotide sequence of a cDNA clone of the horse (Equus caballus) DRA gene

In order to study the evolution of the MHC in mammals, we have begun isolating both class I and class II MHC genes from a domestic horse, Equus caballus , cDNA library. RNA was isolated from peripheral blood cells (PBC) of an A2/A2 homozygous mare (haplotypes identical by descent). The purified RNA...

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Bibliographic Details
Published in:Immunogenetics (New York) 1991, Vol.34 (2), p.136-138
Main Authors: ALBRIGHT, D, BAILEY, E, WOODWARD, J. G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
DNA - analysis
Equus caballus
Fundamental and applied biological sciences. Psychology
Genes. Genome
HLA-DR Antigens - genetics
Horses - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Polymorphism, Genetic
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Summary:In order to study the evolution of the MHC in mammals, we have begun isolating both class I and class II MHC genes from a domestic horse, Equus caballus , cDNA library. RNA was isolated from peripheral blood cells (PBC) of an A2/A2 homozygous mare (haplotypes identical by descent). The purified RNA was sent to Clonetech (Palo Alto, CA) where they performed poly-A selection and then constructed a cDNA library in a lambda gt10 vector. We screened the library (approximately 50,000 plaques) with a mouse Ea cDNA, pEAC11, and isolated two positive clones. Surprisingly, the DNA sequence reveals that the cDNA clone is a partially processed transcript, containing part of the first intron, a complete second intron, but lacking the remaining introns. The cDNA is not full length and is missing exon 1, coding for the leader peptide.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00211427