Comparison between Strains of Human T Lymphotropic Virus Type I Isolated from Inhabitants of the Solomon Islands and Papua New Guinea

Strains of human T lymphotropic virus type I (HTLV-I) isolated from T cell lines (SI-I, SI-3, and SI-5) from three individuals in separate regions of the Solomon Islands were compared with a variant (PNG-I) isolated from a healthy person in Papua New Guinea and a prototype strain from Japan (MT-2)....

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Bibliographic Details
Published in:The Journal of infectious diseases 1991-09, Vol.164 (3), p.443-449
Main Authors: Yanagihara, Richard, Nerurkar, Vivek R., Ajdukiewicz, Andrew B.
Format: Article
Language:English
Subjects:
Adult
AIDS/HIV
Antibodies
Antigens, Viral - immunology
Biological and medical sciences
Blotting, Southern
Cell Line
Cell lines
Cultured cells
DNA
Fluorescent Antibody Technique
Genes, Viral
Genomics
HTLV-I Infections - microbiology
Human T lymphotropic virus 1
Human T-lymphotropic virus 1 - genetics
Human T-lymphotropic virus 1 - immunology
Human T-lymphotropic virus 1 - isolation & purification
Human T-lymphotropic virus 1 - ultrastructure
Human viral diseases
Humans
Infectious diseases
Island life
Major Articles
Medical sciences
Melanesia
Middle Aged
Miscellaneous
Papua New Guinea
Phenotype
Polymerase Chain Reaction
Restriction Mapping
Species Specificity
T lymphocytes
Viral diseases
Viruses
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Description
Summary:Strains of human T lymphotropic virus type I (HTLV-I) isolated from T cell lines (SI-I, SI-3, and SI-5) from three individuals in separate regions of the Solomon Islands were compared with a variant (PNG-I) isolated from a healthy person in Papua New Guinea and a prototype strain from Japan (MT-2). The SI-I, SI-3, and SI-5 cell lines were predominantly CD8+.Expression of gag- and env-encoded virus-specific proteins was detected in SI-I, SI-3, and SI-5 and in MT-2 cells by immunofluorescence and Western immunoblot; gag proteins p19 and p24 were absent in PNG-I cells. HTLV-I gag and pol gene sequences were detected in DNA extracted from SI-I, SI-3, and SI-5 cells by polymerase chain reaction; env sequences were not found in SI-5 cells. Genomic Southern analysis of PstI digests of SI-I, SI-3, and SI-5 DNA exhibited the three moderate-sized fragments typical of prototype HTLV-I. By contrast, PstI digests of PNG-I DNA yielded two larger fragments.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/164.3.443