Distribution and Contents of Free O-Phosphoamino Acids in Animal Tissues

This study was carried out to investigate the distribution and contents of O-phosphoserine (P-Ser), O-phosphothreonine (P-Thr), and O-phosphotyrosine (P-Tyr) as their free forms in animal tissues. After extraction of a tissue sample with trichloroacetic acid, these O-phosphoamino acids were converte...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1991-04, Vol.109 (4), p.577-580
Main Authors: Kataoka, Hiroyuki, Sakiyama, Norihisa, Makita, Masami
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Brain Chemistry
Chickens
Chromatography, Gas
Fishes
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Kidney - chemistry
Liver - chemistry
Male
Mice
Mice, Inbred Strains
Myocardium - chemistry
Organ Specificity
Phosphoserine - analysis
Phosphothreonine - analysis
Phosphotyrosine
Proteins
Species Specificity
Swine
Tyrosine - analogs & derivatives
Tyrosine - analysis
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Summary:This study was carried out to investigate the distribution and contents of O-phosphoserine (P-Ser), O-phosphothreonine (P-Thr), and O-phosphotyrosine (P-Tyr) as their free forms in animal tissues. After extraction of a tissue sample with trichloroacetic acid, these O-phosphoamino acids were converted into their N-isobutoxycarbonyl trimethyl ester derivatives and then quantitated by GLC, with flame photometric detection, on a DB-1701 capillary column. Using this method, nanogram levels of O-phosphoamino acids in tissue samples could be accurately and precisely determined without any interference by coexisting substances. Free P-Ser and P-Thr were widely found in animal tissues, pancreas, spleen, stomach, kidney, liver, and lung containing considerable amounts of these O-phosphoamino acids. On the other hand, free P-Tyr was not detected in any of the tissues investigated in this study.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a123422