The Adhesion of Protein A-Bearing Staphylococcus aureus Organisms to Soluble-Staphylococcal-Antigens-Coated HeLa Cells Mediated by Specific Antibodies

The adhesion of staphylococcal protein A (SpA)-bearing Staphylococcus aureus Cowan I organisms to HeLa cells was enhanced by pretreatment of HeLa cells with staphylococcal extracellular antigens and antibodies to them. The adhesion of HLj, an SpA-poor mutant derived from Cowan I, to HeLa cells was n...

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Published in:MICROBIOLOGY and IMMUNOLOGY 1991, Vol.35(1), pp.1-13
Main Authors: Murai, Miyo, Sakurada, Junji, Seki, Keiko, Ogasawara, Masami, Arai, Yoshiko, Edamura, Keiko, Masuda, Shogo
Format: Article
Language:English
Subjects:
Antibodies, Bacterial - immunology
Antigens, Bacterial - immunology
Bacterial Adhesion - immunology
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
HeLa Cells - immunology
HeLa Cells - microbiology
Hot Temperature
Humans
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Solubility
Staphylococcal Protein A - immunology
Staphylococcus aureus
Staphylococcus aureus - immunology
Staphylococcus aureus - pathogenicity
Virulence
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Summary:The adhesion of staphylococcal protein A (SpA)-bearing Staphylococcus aureus Cowan I organisms to HeLa cells was enhanced by pretreatment of HeLa cells with staphylococcal extracellular antigens and antibodies to them. The adhesion of HLj, an SpA-poor mutant derived from Cowan I, to HeLa cells was not enhanced by the same pretreatment of HeLa cells. Furthermore, the enhanced staphylococcal adhesion was inhibited by soluble SpA. The antigen(s) responsible for the enhanced staphylococcal adhesion was(were) heat stable. Pretreatment of HeLa cells with the mixture of staphylococcal extracellular antigens and antibodies to them also enhanced the adhesion of Cowan I. Similarly the adhesion of Cowan I was enhanced by pretreatment of HeLa cells with extracellular antigens of Pseudomonas aeruginosa and antibodies to them. These results indicated that cell-bound SpA mediated the binding of S. aureus to immune complexes composed of extracellular bacterial products and antibodies to them bound to the surface of HeLa cells, and suggested another role of cell-bound SpA as a co-adhesin with other factors in infections due to S. aureus.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1991.tb01528.x