Immobilization of Lipases on Different Carriers and Their Use in Synthesis of Pentyl Isovalerates

Porcine pancreatic lipase (PPL) and Candida cylindracea lipase (CCL) were immobilized on Celite and Amberlite IRA 938 by deposition from the aqueous solution by the addition of hexane. The influence of the immobilization on the activities of the immobilized lipase derivatives has been studied. The i...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2004-05, Vol.34 (2), p.169-178
Main Authors: Sagiroglu, Ayten, Telefoncu, Azmi
Format: Article
Language:English
Subjects:
Animals
Candida - enzymology
Candida cylindracea
Catalysis
Celite and Amberlite IRA-938
Diatomaceous Earth - chemistry
Enzyme Stability
Enzymes, Immobilized - chemistry
Esterification
Ethers - chemical synthesis
Ethers - chemistry
Hexanes - chemistry
Immobilization
Ion Exchange Resins - chemistry
Kidney - enzymology
Lipase - chemistry
Pentanoic Acids - chemistry
Pentanols - chemistry
Pentyl isovalerates
Porcine pancreatic lipase
Substrate Specificity
Swine
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Summary:Porcine pancreatic lipase (PPL) and Candida cylindracea lipase (CCL) were immobilized on Celite and Amberlite IRA 938 by deposition from the aqueous solution by the addition of hexane. The influence of the immobilization on the activities of the immobilized lipase derivatives has been studied. The immobilized lipases were used in synthesis of pentyl isovalerates. Various reaction parameters affecting the synthesis of pentyl isovalerates were investigated. The reaction rates were compared with the rates of esterification with free lipases. The immobilized lipases were found to be very effective in the esterification reaction. The lipases immobilized on Celite 545 exhibited better operational stabilities than that of immobilized on Amberlite IRA-938.
ISSN:1082-6068
1532-2297
DOI:10.1081/PB-120030875