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Facilitation of Cell Adhesion by Immobilized Dengue Viral Nonstructural Protein 1 (NS1): Arginine-Glycine-Aspartic Acid Structural Mimicry within the Dengue Viral NS1 Antigen
Dengue virus infection causes life-threatening hemorrhagic fever. Increasing evidence implies that dengue viral nonstructural protein 1 (NS1) exhibits a tendency to elicit potentially hazardous autoantibodies, which show a wide spectrum of specificity against extracellular matrix and platelet antige...
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| Published in: | The Journal of infectious diseases 2002-09, Vol.186 (6), p.743-751 |
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| Main Authors: | , , , , , , |
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| container_end_page | 751 |
| container_issue | 6 |
| container_start_page | 743 |
| container_title | The Journal of infectious diseases |
| container_volume | 186 |
| creator | Chang, Hsin-Hou Shyu, Huey-Fen Wang, Yo-Ming Sun, Der-Shan Shyu, Rong-Hwa Tang, Shiao-Shek Huang, Yao-Shine |
| description | Dengue virus infection causes life-threatening hemorrhagic fever. Increasing evidence implies that dengue viral nonstructural protein 1 (NS1) exhibits a tendency to elicit potentially hazardous autoantibodies, which show a wide spectrum of specificity against extracellular matrix and platelet antigens. How NS1 elicits autoantibodies remains unclear. To address the hypothesis that NS1 and matrix proteins may have structural and functional similarity, cell-matrix and cell-NS1 interactions were evaluated using a cell-adhesion assay. The present study showed that dengue NS1 immobilized on coverslips resulted in more cell adhesion than did the control proteins. This cell adhesion was inhibited by peptides containing arginine-glycine-aspartic acid (RGD), a motif important for integrin-mediated cell adhesion. In addition, anti-NS1 antibodies blocked RGD-mediated cell adhesion. Although there is no RGD motif in the NS1 protein sequence, these data indicate that RGD structural mimicry exists within the NS1 antigen |
| doi_str_mv | 10.1086/342600 |
| format | article |
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Increasing evidence implies that dengue viral nonstructural protein 1 (NS1) exhibits a tendency to elicit potentially hazardous autoantibodies, which show a wide spectrum of specificity against extracellular matrix and platelet antigens. How NS1 elicits autoantibodies remains unclear. To address the hypothesis that NS1 and matrix proteins may have structural and functional similarity, cell-matrix and cell-NS1 interactions were evaluated using a cell-adhesion assay. The present study showed that dengue NS1 immobilized on coverslips resulted in more cell adhesion than did the control proteins. This cell adhesion was inhibited by peptides containing arginine-glycine-aspartic acid (RGD), a motif important for integrin-mediated cell adhesion. In addition, anti-NS1 antibodies blocked RGD-mediated cell adhesion. Although there is no RGD motif in the NS1 protein sequence, these data indicate that RGD structural mimicry exists within the NS1 antigen</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/342600</identifier><identifier>PMID: 12198607</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies ; Antibodies, Viral - immunology ; Antigens, Viral - chemistry ; Antigens, Viral - immunology ; Antigens, Viral - metabolism ; Biological and medical sciences ; Cell Adhesion ; Cell Adhesion Molecules - chemistry ; Cell Adhesion Molecules - immunology ; Cell Adhesion Molecules - metabolism ; Cells, Cultured ; Dengue ; Dengue - immunology ; Dengue - pathology ; Dengue - virology ; Dengue Virus - chemistry ; Dengue Virus - immunology ; Dengue Virus - metabolism ; Endothelial cells ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoglobulins ; Infections ; Japanese encephalitis viruses ; Major Article ; Microbiology ; Molecular Mimicry ; Oligopeptides - immunology ; Oligopeptides - metabolism ; Rabbits ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Tropical medicine ; Viral Nonstructural Proteins - chemistry ; Viral Nonstructural Proteins - immunology ; Viral Nonstructural Proteins - metabolism ; Virology ; Viruses</subject><ispartof>The Journal of infectious diseases, 2002-09, Vol.186 (6), p.743-751</ispartof><rights>Copyright 2002 Infectious Diseases Society of America</rights><rights>2002 by the Infectious Diseases Society of America 2002</rights><rights>2003 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Sep 15, 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-f9e30ba7d0ef88454beeb40993966306a7983976b5d8c04c1c001e35c3ae79523</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30085695$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30085695$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13911530$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12198607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Hsin-Hou</creatorcontrib><creatorcontrib>Shyu, Huey-Fen</creatorcontrib><creatorcontrib>Wang, Yo-Ming</creatorcontrib><creatorcontrib>Sun, Der-Shan</creatorcontrib><creatorcontrib>Shyu, Rong-Hwa</creatorcontrib><creatorcontrib>Tang, Shiao-Shek</creatorcontrib><creatorcontrib>Huang, Yao-Shine</creatorcontrib><title>Facilitation of Cell Adhesion by Immobilized Dengue Viral Nonstructural Protein 1 (NS1): Arginine-Glycine-Aspartic Acid Structural Mimicry within the Dengue Viral NS1 Antigen</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><addtitle>The Journal of Infectious Diseases</addtitle><description>Dengue virus infection causes life-threatening hemorrhagic fever. Increasing evidence implies that dengue viral nonstructural protein 1 (NS1) exhibits a tendency to elicit potentially hazardous autoantibodies, which show a wide spectrum of specificity against extracellular matrix and platelet antigens. How NS1 elicits autoantibodies remains unclear. To address the hypothesis that NS1 and matrix proteins may have structural and functional similarity, cell-matrix and cell-NS1 interactions were evaluated using a cell-adhesion assay. The present study showed that dengue NS1 immobilized on coverslips resulted in more cell adhesion than did the control proteins. This cell adhesion was inhibited by peptides containing arginine-glycine-aspartic acid (RGD), a motif important for integrin-mediated cell adhesion. In addition, anti-NS1 antibodies blocked RGD-mediated cell adhesion. Although there is no RGD motif in the NS1 protein sequence, these data indicate that RGD structural mimicry exists within the NS1 antigen</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens, Viral - chemistry</subject><subject>Antigens, Viral - immunology</subject><subject>Antigens, Viral - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Cell Adhesion Molecules - chemistry</subject><subject>Cell Adhesion Molecules - immunology</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cells, Cultured</subject><subject>Dengue</subject><subject>Dengue - immunology</subject><subject>Dengue - pathology</subject><subject>Dengue - virology</subject><subject>Dengue Virus - chemistry</subject><subject>Dengue Virus - immunology</subject><subject>Dengue Virus - metabolism</subject><subject>Endothelial cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Infections</subject><subject>Japanese encephalitis viruses</subject><subject>Major Article</subject><subject>Microbiology</subject><subject>Molecular Mimicry</subject><subject>Oligopeptides - immunology</subject><subject>Oligopeptides - metabolism</subject><subject>Rabbits</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Tropical medicine</subject><subject>Viral Nonstructural Proteins - chemistry</subject><subject>Viral Nonstructural Proteins - immunology</subject><subject>Viral Nonstructural Proteins - metabolism</subject><subject>Virology</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkt2O0zAQhSMEYssCbwDyIoHgIjCOYzvmLhT2B5YFqYBWe2M5jtO6JHGxHUF5KJ6RVK220kqIq9H4fD4j-0ySPMTwEkPBXpE8YwC3kgmmhKeMYXI7mQBkWYoLIQ6SeyEsASAnjN9NDnCGRcGAT5I_x0rb1kYVreuRa9DUtC0q64UJm4Nqjc66zlUj8tvU6K3p54NB36xXLbpwfYh-0HHYdJ-9i8b2CKPnFzP84jUq_dz2tjfpSbvWm1qGlfLRalRqW6PZ_upH21nt1-injYvRIS7MjUEzjMo-2rnp7yd3GtUG82BXD5Ovx---TE_T808nZ9PyPNU05zFthCFQKV6DaYoip3llTJWDEEQwRoApLgoiOKtoXWjINdYA2BCqiTJc0IwcJs-2vivvfgwmRNnZoMevUb1xQ5A8A5JTLP4L4oJjQgkZwSc3wKUbfD8-QmYZEYAxK_Zu2rsQvGnkyttO-bXEIDc5y23OI_h45zZUnan32C7YEXi6A1TQqm286rUNe44IPG7Kxuhoy7lh9e9hj7bMMkTnrykCUFAm6KinW92GaH5d68p_l4wTTuXp5ZUsruDyTcbfyw_kLwFk0NI</recordid><startdate>20020915</startdate><enddate>20020915</enddate><creator>Chang, Hsin-Hou</creator><creator>Shyu, Huey-Fen</creator><creator>Wang, Yo-Ming</creator><creator>Sun, Der-Shan</creator><creator>Shyu, Rong-Hwa</creator><creator>Tang, Shiao-Shek</creator><creator>Huang, Yao-Shine</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020915</creationdate><title>Facilitation of Cell Adhesion by Immobilized Dengue Viral Nonstructural Protein 1 (NS1): Arginine-Glycine-Aspartic Acid Structural Mimicry within the Dengue Viral NS1 Antigen</title><author>Chang, Hsin-Hou ; Shyu, Huey-Fen ; Wang, Yo-Ming ; Sun, Der-Shan ; Shyu, Rong-Hwa ; Tang, Shiao-Shek ; Huang, Yao-Shine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c547t-f9e30ba7d0ef88454beeb40993966306a7983976b5d8c04c1c001e35c3ae79523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens, Viral - chemistry</topic><topic>Antigens, Viral - immunology</topic><topic>Antigens, Viral - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Cell Adhesion Molecules - chemistry</topic><topic>Cell Adhesion Molecules - immunology</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cells, Cultured</topic><topic>Dengue</topic><topic>Dengue - immunology</topic><topic>Dengue - pathology</topic><topic>Dengue - virology</topic><topic>Dengue Virus - chemistry</topic><topic>Dengue Virus - immunology</topic><topic>Dengue Virus - metabolism</topic><topic>Endothelial cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoglobulins</topic><topic>Infections</topic><topic>Japanese encephalitis viruses</topic><topic>Major Article</topic><topic>Microbiology</topic><topic>Molecular Mimicry</topic><topic>Oligopeptides - immunology</topic><topic>Oligopeptides - metabolism</topic><topic>Rabbits</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Tropical medicine</topic><topic>Viral Nonstructural Proteins - chemistry</topic><topic>Viral Nonstructural Proteins - immunology</topic><topic>Viral Nonstructural Proteins - metabolism</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Hsin-Hou</creatorcontrib><creatorcontrib>Shyu, Huey-Fen</creatorcontrib><creatorcontrib>Wang, Yo-Ming</creatorcontrib><creatorcontrib>Sun, Der-Shan</creatorcontrib><creatorcontrib>Shyu, Rong-Hwa</creatorcontrib><creatorcontrib>Tang, Shiao-Shek</creatorcontrib><creatorcontrib>Huang, Yao-Shine</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Hsin-Hou</au><au>Shyu, Huey-Fen</au><au>Wang, Yo-Ming</au><au>Sun, Der-Shan</au><au>Shyu, Rong-Hwa</au><au>Tang, Shiao-Shek</au><au>Huang, Yao-Shine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Facilitation of Cell Adhesion by Immobilized Dengue Viral Nonstructural Protein 1 (NS1): Arginine-Glycine-Aspartic Acid Structural Mimicry within the Dengue Viral NS1 Antigen</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2002-09-15</date><risdate>2002</risdate><volume>186</volume><issue>6</issue><spage>743</spage><epage>751</epage><pages>743-751</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Dengue virus infection causes life-threatening hemorrhagic fever. Increasing evidence implies that dengue viral nonstructural protein 1 (NS1) exhibits a tendency to elicit potentially hazardous autoantibodies, which show a wide spectrum of specificity against extracellular matrix and platelet antigens. How NS1 elicits autoantibodies remains unclear. To address the hypothesis that NS1 and matrix proteins may have structural and functional similarity, cell-matrix and cell-NS1 interactions were evaluated using a cell-adhesion assay. The present study showed that dengue NS1 immobilized on coverslips resulted in more cell adhesion than did the control proteins. This cell adhesion was inhibited by peptides containing arginine-glycine-aspartic acid (RGD), a motif important for integrin-mediated cell adhesion. In addition, anti-NS1 antibodies blocked RGD-mediated cell adhesion. Although there is no RGD motif in the NS1 protein sequence, these data indicate that RGD structural mimicry exists within the NS1 antigen</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>12198607</pmid><doi>10.1086/342600</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of infectious diseases, 2002-09, Vol.186 (6), p.743-751 |
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| source | JSTOR Archival Journals and Primary Sources Collection; Oxford Journals Online |
| subjects | Amino Acid Sequence Animals Antibodies Antibodies, Viral - immunology Antigens, Viral - chemistry Antigens, Viral - immunology Antigens, Viral - metabolism Biological and medical sciences Cell Adhesion Cell Adhesion Molecules - chemistry Cell Adhesion Molecules - immunology Cell Adhesion Molecules - metabolism Cells, Cultured Dengue Dengue - immunology Dengue - pathology Dengue - virology Dengue Virus - chemistry Dengue Virus - immunology Dengue Virus - metabolism Endothelial cells Fundamental and applied biological sciences. Psychology Humans Immunoglobulins Infections Japanese encephalitis viruses Major Article Microbiology Molecular Mimicry Oligopeptides - immunology Oligopeptides - metabolism Rabbits Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Tropical medicine Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - immunology Viral Nonstructural Proteins - metabolism Virology Viruses |
| title | Facilitation of Cell Adhesion by Immobilized Dengue Viral Nonstructural Protein 1 (NS1): Arginine-Glycine-Aspartic Acid Structural Mimicry within the Dengue Viral NS1 Antigen |
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