EFFECT OF VACCINATIONS WITH RECOMBINANT FUSION PROTEINS ON ANCYLOSTOMA CANINUM HABITAT SELECTION IN THE CANINE INTESTINE

• Published in: The Journal of parasitology 2002-08, Vol.88 (4), p.684-690
• Main Authors: Hotez, Peter J, Ashcom, James, Bin, Zhan, Bethony, Jeffrey, Williamson, Angela, Hawdon, John M, Jianjun, Feng, Dobardzic, Azra, Rizo, Ivania, Bolden, Janelle, Jin, Qun, Yan, Wang, Dobardzic, Reshad, Chung-Debose, Sophia, Crowell, Melissa, Datu, Bennett, Delaney, Angela, Dragonovski, Dilyan, Jiang, Yang, Yueyuan, Liu, Ghosh, Kashinath, Loukas, Alex, Brandt, Walter, Russell, Philip K, Zook, Bernard C
• Format: Article
• Language: English
• Subjects: Ancylostoma; Ancylostomiasis - immunology; Ancylostomiasis - parasitology; Animals; Antibodies; Antibodies, Helminth - biosynthesis; Antigens; Antigens, Helminth - administration & dosage; Antigens, Helminth - immunology; Biological and medical sciences; Canines; Colon; Dogs; Experimental helminthic diseases. Models; Female; Fundamental and applied biological sciences. Psychology; Helminthic diseases; Immunization; IMMUNOLOGY; Infectious diseases; Intestines - parasitology; Invertebrates; Male; Medical sciences; Nemathelminthia. Plathelmintha; Parasite Egg Count; Parasites; Parasitic diseases; Parasitology; Recombinant Fusion Proteins - administration & dosage; Recombinant Fusion Proteins - immunology; Small intestine; Vaccination; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/0022-3395(2002)088[0684:EOVWRF]2.0.CO;2

Laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. The vaccinated dogs were then challenged orally with 400 third-stage infective larvae (L3) of the canine hookworm, Ancylostoma caninum. The 3 A. caninum antigens selected were Ac-TMP, an adult-specific secreted tissue inhibitor of metalloproteases; Ac-AP, an adult-specific secreted factor Xa serine protease inhibitor anticoagulant; and Ac-ARR-1, a cathepsin D–like aspartic protease. Each of the 3 groups comprised 6 male beagles (8 ± 1 wk of age). A fourth group comprised control dogs injected with alum. All of the dogs vaccinated with Ac-TMP or Ac-APR-1 exhibited a vigorous antigen-specific antibody response, whereas only a single dog vaccinated with Ac-AP developed an antibody response. Dogs with circulating antibody responses exhibited 4.5–18% reduction in the numbers of adult hookworms recovered from the small intestines at necropsy, relative to alum-injected dogs. In contrast, there was a concomitant increase in the number of adult hookworms recovered from the colon. The increase in colonic hookworms was as high as 500%, relative to alum-injected dogs. Female adult hookworms were more likely to migrate into the colon than were males. Anti-enzyme and anti-enzyme inhibitor antibodies correlated with an alteration in adult hookworm habitat selection in the canine gastrointestinal tract.