Insertion of an exogenous domain in the adenovirus type 2 fiber globular region

Adenoviruses have been used for gene therapy or immunization due to their ability to efficiently infect a broad range of cells and tissues. These applications as well as specificity could be improved further by redirecting binding of the virus to specific cell types. In this regard, modification of...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-08, Vol.296 (4), p.897-903
Main Authors: Zerbini, L.F, Libermann, T.A, Ventura, A.M
Format: Article
Language:English
Subjects:
Adenoviridae - chemistry
Adenoviridae - metabolism
Adenovirus
Capsid - chemistry
Cell Line
Epitopes
Fiber protein
Genome, Viral
HIV Envelope Protein gp120 - metabolism
Human immunodeficiency virus 1
Human immunodeficiency virus 2
Knob domain
Ligands
Models, Biological
Polymerase Chain Reaction
Precipitin Tests
Protein Binding
Protein Biosynthesis
Protein Structure, Tertiary
Recombination, Genetic
Transcription, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adenoviruses have been used for gene therapy or immunization due to their ability to efficiently infect a broad range of cells and tissues. These applications as well as specificity could be improved further by redirecting binding of the virus to specific cell types. In this regard, modification of viral genes encoding coat proteins is an option to achieve improvement in retargeting. In this report, we describe a substitution in the adenovirus type 2 fiber globular region by the 44 amino acid C4 domain of human immunodeficiency virus type 1 gp120. In vitro translation analysis and immunoprecipitation assays show that the incorporation of the C4 domain into the fiber protein does not ablate its trimerization property and demonstrates the availability of the C4 epitope for interaction with monoclonal anti-C4 antibody. The recombinant adenovirus containing this modified fiber was also characterized by immunoprecipitation with the same antibody, showing the viability of such kind of modification.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02005-3