Reversed-phase high-performance liquid chromatography method for the determination of bemegride in serum and brain tissue: Pharmacokinetics and brain distribution of an intraperitoneal subconvulsive dose in rats

A simple and rapid HPLC method has been developed for the quantification of bemegride in serum and brain tissue, using p-methylphenobarbital as an internal standard. Serum and brain tissue homogenate samples were extracted with ethyl acetate and the evaporated and redissolved extracts injected into...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1991, Vol.9 (2), p.177-182
Main Authors: Soto-Otero, R., Mendez-Alvarez, E., Sierra-Paredes, G., Galanvalientiente, J., Aguilar-Veiga, E., Sierra-Marcuño, G.
Format: Article
Language:English
Subjects:
Analysis
Animals
bemegride
Bemegride - analysis
Bemegride - blood
Bemegride - pharmacokinetics
Biological and medical sciences
Brain Chemistry
brain tissue
Chromatography, High Pressure Liquid
General pharmacology
Injections, Intraperitoneal
Male
Medical sciences
pharmacokinetics
Pharmacology. Drug treatments
Rats
Rats, Inbred Strains
Reversed-phase HPLC
serum
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Summary:A simple and rapid HPLC method has been developed for the quantification of bemegride in serum and brain tissue, using p-methylphenobarbital as an internal standard. Serum and brain tissue homogenate samples were extracted with ethyl acetate and the evaporated and redissolved extracts injected into a reversed-phase column. The compounds were eluted with an acetonitrile—phosphate buffer mixture and monitored at 200 nm. A linear response was obtained in the range 1–40 μg ml −1 for serum and 1–40 μg g −1 for brain tissue. Within-day and between-day precisions were
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(91)80141-U