The first non-turnover voltammetric response from a molybdenum enzyme: direct electrochemistry of dimethylsulfoxide reductase from Rhodobacter capsulatus

The first direct voltammetric response from a molybdenum enzyme under non-turnover conditions is reported. Cyclic voltammetry of dimethylsulfoxide reductase from Rhodobacter capsulatus reveals a reversible Mo(VI/V) response at +161 mV followed by a reversible Mo(V/IV) response at -102 mV versus NHE...

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Bibliographic Details
Published in:Journal of biological inorganic chemistry 2002-09, Vol.7 (7-8), p.879-883
Main Authors: Aguey-Zinsou, Kondo-François, Bernhardt, Paul V, McEwan, Alastair G, Ridge, Justin P
Format: Article
Language:English
Subjects:
Electrochemistry
Electrodes
Hydrogen-Ion Concentration
Indicators and Reagents
Iron-Sulfur Proteins
Molybdenum - chemistry
Oxidoreductases - chemistry
Rhodobacter capsulatus - enzymology
Spectrophotometry, Ultraviolet
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Summary:The first direct voltammetric response from a molybdenum enzyme under non-turnover conditions is reported. Cyclic voltammetry of dimethylsulfoxide reductase from Rhodobacter capsulatus reveals a reversible Mo(VI/V) response at +161 mV followed by a reversible Mo(V/IV) response at -102 mV versus NHE at pH 8. The higher potential couple exhibits a pH dependence consistent with protonation upon reduction to the Mo(V) state and we have determined the p K(a) for this semi-reduced species to be 9.0. The lower potential couple is pH independent within the range 5
ISSN:0949-8257
1432-1327
DOI:10.1007/s00775-002-0374-y