Quantitation of platelet adhesion to Ti and DLC-coated Ti in vitro using (125)I-labeled platelets

Measurement of platelet adhesion in vitro is a good indicator of its reactivity to implant devices in vivo. Platelets were labeled with I-125 without affecting its normal morphology and function and the labeled platelets were suspended in platelet poor plasma and exposed to Ti and diamond like carbo...

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Bibliographic Details
Published in:Biomolecular engineering 2002-08, Vol.19 (2-6), p.251-253
Main Authors: Krishnan, L K, Varghese, N, Muraleedharan, C V, Bhuvaneshwar, G S, Derangère, F, Sampeur, Y, Suryanarayanan, R
Format: Article
Language:English
Subjects:
Blood Platelets - diagnostic imaging
Blood Platelets - physiology
Carbon
Cell Adhesion
Cell Count
Coated Materials, Biocompatible
Diamond
Humans
In Vitro Techniques
Iodine Radioisotopes
Isotope Labeling - methods
Materials Testing - methods
Platelet Adhesiveness
Radionuclide Imaging
Titanium
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Summary:Measurement of platelet adhesion in vitro is a good indicator of its reactivity to implant devices in vivo. Platelets were labeled with I-125 without affecting its normal morphology and function and the labeled platelets were suspended in platelet poor plasma and exposed to Ti and diamond like carbon-coated (DLC) Ti discs, under dynamic conditions, using a parallel plate flow chamber. The test materials were washed, dried, exposed to a phosphor screen and scanned to get the images. The number of platelets that adhered to Ti was found to be higher than those that adhered to DLC coated Ti sample, irrespective of the shear stress which was varied between 2 and 16 dynes/cm(2).
ISSN:1389-0344
DOI:10.1016/S1389-0344(02)00029-1