Cell orientation and cytoskeleton organisation on ground titanium surfaces

A stable connection between the biomaterial surface and the surrounding tissue is one of the most important prerequisites for the long-term success of implants. Therefore, a strong adhesion of the cells on the biomaterial surface is required. Beside the surface composition the surface topography inf...

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Bibliographic Details
Published in:Biomolecular engineering 2002-08, Vol.19 (2), p.233-237
Main Authors: Eisenbarth, E, Linez, P, Biehl, V, Velten, D, Breme, J, Hildebrand, H.F
Format: Article
Language:English
Subjects:
3T3 Cells - physiology
3T3 Cells - ultrastructure
Animals
Aorta - ultrastructure
Biocompatible Materials - chemistry
Biocompatible Materials - classification
Cattle
Cell Adhesion
Cell Line
Cell Movement
Cell orientation
Cell Polarity
Cercopithecus aethiops
Cytoskeleton - ultrastructure
Endothelium, Vascular - physiology
Endothelium, Vascular - ultrastructure
Fibroblasts - physiology
Fibroblasts - ultrastructure
Gingiva - ultrastructure
Materials Testing - methods
Mice
Sensitivity and Specificity
Surface Properties
Surface topography
Titanium - chemistry
Titanium - classification
Vero Cells
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Summary:A stable connection between the biomaterial surface and the surrounding tissue is one of the most important prerequisites for the long-term success of implants. Therefore, a strong adhesion of the cells on the biomaterial surface is required. Beside the surface composition the surface topography influences the properties of the adherent cells. The quality of the connection between the cell and the biomaterial is-among other factors-determined by the dimensions of the surface topography. Osteoblasts and fibroblast-like cells in contact with a ground biomaterial surface spread in the direction of the surface structures. These aligned cells provide a more favourable adhesion behaviour than a spherically shaped cell. To determine the influence of the surface structure on the cell alignment and cytoskeleton organisation or arrangement, substrate discs of cp-titanium were ground, producing different roughness of the substrates.The oriented cells had a higher density of focal contacts when they were in contact with the edges of the grooves and showed a better organisation of the cytoskeleton and stronger actin fibres. These changes of the aligned cells depend on the peak to valley height of the surface structures.
ISSN:1389-0344
1878-559X
DOI:10.1016/S1389-0344(02)00028-X