Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus

The complete genomic sequence of canine calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other calicivirus strain with a completel...

Full description

Saved in:
Bibliographic Details
Published in:Virus genes 2002-08, Vol.25 (1), p.67-73
Main Authors: Matsuura, Yuichi, Tohya, Yukinobu, Nakamura, Kazuya, Shimojima, Masayuki, Roerink, Frank, Mochizuki, Masami, Takase, Kozo, Akashi, Hiroomi, Sugimura, Takaaki
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Blotting, Northern
Caliciviridae - classification
Caliciviridae - genetics
Conserved Sequence
DNA, Viral - analysis
Dogs
Genome, Viral
Molecular Sequence Data
Open Reading Frames
Phylogeny
Poly A - chemistry
Viral Nonstructural Proteins - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The complete genomic sequence of canine calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other calicivirus strain with a completely known sequence. There were three open reading frames (ORF1, nt 12-5801; ORF2, nt 5805-7880; and ORF3, nt 7877-8278). ORF1 encoded a polyprotein (calculated Mr of 214,802) which had the conserved motifs of non-structural proteins of other caliciviruses and picornaviruses. Regions containing characteristic motifs in the non-structural polyprotein of CaCV showed highest similarity with those of the species Feline calicivirus and Vesicular exanthema of swine virus in the genus Vesivirus. Phylogenic analysis indicated that CaCV formed a distinct branch within the genus. Our results strongly suggested that CaCV is a new species in the genus Vesivirus.
ISSN:0920-8569
1572-994X
DOI:10.1023/A:1020174225622