Oxytocin stimulates secretion of prostaglandin F(2alpha) from endometrial cells of swine in the presence of progesterone

Oxytocin (OT) stimulates endometrial secretion of prostaglandin (PG) F(2 alpha) during corpus luteum regression in swine but there is differential responsiveness to OT among endometrial cell types. To determine if progesterone influenced responsiveness of luminal epithelial, glandular epithelial, an...

Full description

Saved in:
Bibliographic Details
Published in:Domestic animal endocrinology 2002-10, Vol.23 (3), p.435-445
Main Authors: Carnahan, K G, Uzumcu, M, Hu, J, Sample, G L, Braileanu, G T, Mirando, M A
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Dinoprost - secretion
Endometrium - drug effects
Endometrium - secretion
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Female
Luteolysis - physiology
Oxytocin - pharmacology
Progesterone - pharmacology
Stromal Cells - drug effects
Stromal Cells - metabolism
Swine - physiology
Type C Phospholipases - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oxytocin (OT) stimulates endometrial secretion of prostaglandin (PG) F(2 alpha) during corpus luteum regression in swine but there is differential responsiveness to OT among endometrial cell types. To determine if progesterone influenced responsiveness of luminal epithelial, glandular epithelial, and stromal cells to 100 nM OT during luteolysis in swine, cells were isolated from endometrium of 15 gilts by differential enzymatic digestion and sieve filtration on day 16 postestrus and cultured continuously in the presence of 0, 10 or 100 nM progesterone. For phospholipase C (PLC) activity and PGF(2 alpha) secretion, stromal cells were most responsive to OT (P
ISSN:0739-7240