Thy-1 and T-Cell Receptor Antigen Expression in Mycosis Fungoides and Benign Inflammatory Dermatoses

• Published in: JNCI : Journal of the National Cancer Institute 1991-08, Vol.83 (15), p.1088-1092
• Main Authors: Fivenson, David P., Rheins, Lawrence A., Nordlund, James J., Pomaranski, Mark, Douglass, Margaret C., Krull, Edward A.
• Format: Article
• Language: English
• Subjects: Antigens, Surface - physiology; Biological and medical sciences; Biopsy; Dermatitis - immunology; Dermatitis - metabolism; Dermatitis - pathology; Hematologic and hematopoietic diseases; Humans; Immunohistochemistry; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphocytes - immunology; Lymphocytes - metabolism; Medical sciences; Mycosis Fungoides - immunology; Mycosis Fungoides - metabolism; Mycosis Fungoides - pathology; Receptors, Antigen, T-Cell - physiology; Receptors, Antigen, T-Cell, alpha-beta; Receptors, Antigen, T-Cell, gamma-delta; Skin - cytology; T-Lymphocytes - immunology; Thy-1 Antigens
• ISSN: 0027-8874; 1460-2105
• DOI: 10.1093/jnci/83.15.1088

we have studied human Thy-1 and T-cells receptor (TCR) antigen expression in mycosis fungoides and benign inflammatory dermatoses. The study included 24 biopsy specimens from 21 patients with mycosis fungoides (ninepatch stage from eight patients, 13 plaque stage from 11 patients, and two tumor stage from two patients), six specimens from five patients with premycotic parapsoriasis (pre-mycosis fungoides), three specimens from three patients with lichen planus, 11 specimens from 11 patients with lupus erythematosus, 13 specimens from 13 patients with dermatitis, six specimens from six patients with drug eruptions, nine normal skin specimens from nine subjects, and three specimens from three patients with small plaque (benign) parapsoriasis. Immuno-peroxidase studies using the avidin-biotin complex technique on serial frozen sections were performed. Primary antibodies were anti-human Thy-1, anti-α heterodimer of the TCR, anti-β heterodimer of the TCR, and anti-5 heterodimer of the TCR. An extensive dendritic network of Thy-1+ cells was seen in all cases of mycosis fungoides. Epidermotropic cells were Thy-1 negative, and Thy-1 was expressed perivascularly in normal individuals and patients as previously reported. Epidermal r//δ cells were seen only in mycosis fungoides, where up to 60% of the epidermal lymphocytes expressed this TCR. The increased numbers of Thy-1 and r//δ T cells in mycosis fungoides were statistically significant when compared with normal skin or benign inflammatory dermatoses. The role of these dendritic dermal Thy-1+ cells and epidermal r//δ T cells in mycosis fungoides is unclear. The significant numbers of these potentially immunomodulating cells that were seen suggest that they are involved in the pathogenesis of mycosis fungoides. [J Natl Cancer Inst 83: 1088–1092, 1991]