Tetraethylammonium transport by snake renal brush-border membrane vesicles

Transport of tetraethylammonium (TEA) by snake (Thamnophis spp.) renal brush-border membrane vesicles (BBMV) was studied. An outwardly directed proton gradient (pH 6.0 in, pH 7.5 out) stimulated uptake of TEA into BBMV and supported concentrative accumulation. Uptake of radioactively labeled TEA was...

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Bibliographic Details
Published in:Pflügers Archiv 1991-05, Vol.418 (4), p.325-332
Main Authors: DANTZLER, W. H, WRIGHT, S. H, BROKL, O. H
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Transport - drug effects
Biological Transport - physiology
Carrier Proteins - physiology
Cell Membrane - drug effects
Cell Membrane - physiology
Cell Membrane - ultrastructure
Female
Fundamental and applied biological sciences. Psychology
Kidney - physiology
Kidney - ultrastructure
Male
Membrane Potentials - drug effects
Membrane Potentials - physiology
Microvilli - physiology
Microvilli - ultrastructure
Snakes - metabolism
Snakes - physiology
Sodium - pharmacology
Sodium-Hydrogen Exchangers
Tetraethylammonium
Tetraethylammonium Compounds - metabolism
Tetraethylammonium Compounds - pharmacokinetics
Vertebrates: urinary system
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Summary:Transport of tetraethylammonium (TEA) by snake (Thamnophis spp.) renal brush-border membrane vesicles (BBMV) was studied. An outwardly directed proton gradient (pH 6.0 in, pH 7.5 out) stimulated uptake of TEA into BBMV and supported concentrative accumulation. Uptake of radioactively labeled TEA was also stimulated by outwardly directed gradients of unlabeled TEA and choline. The initial rate of TEA uptake was a saturable process that was adequately described by Michaelis-Menten kinetics. TEA uptake was not influenced by changes in the electrical potential difference across the membranes. Although uptake of TEA was stimulated by an outwardly directed Na+ gradient and inhibited by an inwardly directed Na+ gradient, these effects were probably secondary to the generation of proton gradients via a Na+/H+ exchanger demonstrated in these same BBMV. In agreement with previous studies with intact snake renal tubules, the present results indicate that TEA transport across the brush-border membrane involves electroneutral countertransport for protons or organic cations.
ISSN:0031-6768
1432-2013
DOI:10.1007/BF00550869