Laminin-1 increases motility, path-searching, and process dynamism of rat and mouse Muller glial cells in vitro: Implication of relationship between cell behavior and formation of retinal morphology

Spatial correlation was observed between the localization of laminin‐1 at the inner limiting membrane (ILM) and extensive Muller glial process arborization in the same area, as demonstrated by immunolabeling of Muller glial processes and laminin‐1 in rat retinae in situ. To test if this spatial corr...

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Published in:Cell motility and the cytoskeleton 2002-11, Vol.53 (3), p.203-213
Main Authors: Méhes, Előd, Czirók, András, Hegedüs, Balázs, Vicsek, Tamás, Jancsik, Veronika
Format: Article
Language:English
Subjects:
Animals
cell culture
Cell Movement - physiology
Cells, Cultured
cytoskeleton
extracellular matrix
Immunohistochemistry
Laminin - metabolism
Mice
Mice, Inbred C57BL
Microscopy, Video
Neuroglia - cytology
Neuroglia - metabolism
Rats
Rats, Wistar
Retina - cytology
statistical analysis
videomicroscopy
Vimentin - metabolism
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Summary:Spatial correlation was observed between the localization of laminin‐1 at the inner limiting membrane (ILM) and extensive Muller glial process arborization in the same area, as demonstrated by immunolabeling of Muller glial processes and laminin‐1 in rat retinae in situ. To test if this spatial correlation is due to a functional relationship, we investigated the impact of laminin‐1 on the motility of cultured primary rat and mouse retinal Muller glial cells by statistical analysis of computer‐controlled videomicroscopic time‐lapse images. We demonstrate that laminin‐1 increases motility and path‐searching activity of Muller cells in vitro and it also enhances the cells' process formation/withdrawal dynamism. The increase in path‐searching activity and cell process dynamism indicates that there is a functional relationship between laminin‐1 and Muller glial cells presumably involving signaling towards the cytoskeleton. We hypothesize that laminin‐1 is involved in process arborization of Muller cells at the vitread border of the retina resulting in the formation of the functional barrier made up of Muller glial endfeet. Cell Motil. Cytoskeleton 53:203–213, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:0886-1544
1097-0169
DOI:10.1002/cm.10062