Polymorphism analysis of the internal transcribed spacer and small subunit of ribosomal RNA genes of Leishmania mexicana

Leishmania mexicana causes a wide spectrum of clinical diseases. In spite of the variety of clinical forms, no data exist regarding genetic polymorphism of L. mexicana. We analyzed the polymorphism of the internal transcribed spacer (ITS) and the small subunit rRNA genes of 3 reference strains and 2...

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Bibliographic Details
Published in:Parasitology research (1987) 2002-10, Vol.88 (10), p.918-925
Main Authors: BERZUNZA-CRUZ, Miriam, CABRERA, Nallely, CRIPPA-ROSSI, Marco, CABRERA, Tayde Sosa, PEREZ-MONTFORT, Ruy, BECKER, Ingeborg
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
DNA Restriction Enzymes - metabolism
DNA, Kinetoplast - analysis
DNA, Protozoan - genetics
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
DNA, Ribosomal Spacer - isolation & purification
Fundamental and applied biological sciences. Psychology
Genes, Protozoan
Genes, rRNA
Genetics of eukaryotes. Biological and molecular evolution
Humans
Leishmania mexicana - genetics
Leishmaniasis - classification
Leishmaniasis - genetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Population genetics, reproduction patterns
Protozoa
Restriction Mapping
RNA, Ribosomal - genetics
Transcription, Genetic
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Summary:Leishmania mexicana causes a wide spectrum of clinical diseases. In spite of the variety of clinical forms, no data exist regarding genetic polymorphism of L. mexicana. We analyzed the polymorphism of the internal transcribed spacer (ITS) and the small subunit rRNA genes of 3 reference strains and 24 Mexican isolates of L. mexicana, by means of polymerase chain reaction and subsequent digestion by restriction enzymes. All strains of L. mexicana had invariant patterns for both the ITS and the small subunit of rRNA genes. Leishmania amazonensis and Leishmania venezuelensis displayed polymorphism only in the ITS. The high degree of identity of this region was confirmed by sequencing DNA from three L. mexicana isolates. There was almost complete identity of the sequence for the ITS region of L. venezuelensis and that of strains of Leishmania major, suggesting that these species may be more closely related than previously thought.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-002-0672-x