Differential effect of heme oxygenase-1 in endothelial and smooth muscle cell cycle progression

• Published in: Biochemical and biophysical research communications 2002-09, Vol.296 (5), p.1077-1082
• Main Authors: Li Volti, Giovanni, Wang, Jishi, Traganos, Frank, Kappas, Attallah, Abraham, Nader G
• Format: Article
• Language: English
• Subjects: Arterial remodeling; Cell Cycle; Cell Division; Cell proliferation; Cells, Cultured; DNA - analysis; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - enzymology; Enzyme Inhibitors - pharmacology; Heme oxygenase; Heme Oxygenase (Decyclizing) - antagonists & inhibitors; Heme Oxygenase (Decyclizing) - physiology; Heme Oxygenase-1; Humans; Membrane Proteins; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - enzymology; Vascular wall; Vinblastine - pharmacology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(02)02054-5

Arterial remodeling in response to pathological insult is a complex process that depends in part on the balance between vascular cell apoptosis and proliferation. Studies in experimental models suggest that HO-1 mediates neointimal formation while limiting lumen stenosing, indicating a differential effect on vascular endothelial (EC) and smooth muscle cells (SMC). We investigated the effect of HO-1 expression on cell cycle progression in EC and SMC. The addition of SnMP (10 μM), an inhibitor of HO activity, to EC or SMC for 24 h, resulted in significant abnormalities in DNA distribution and cell cycle progression compared to cells treated with the HO-1 inducers, heme (10 μM) or SnCl 2 (10 μM). SnMP increased G 1 phase and decreased S and G 2/M phases in EC while heme or SnCl 2 decreased G 1 phase, but increased S and G 2/M phases ( p