Molecular phylogenetic analysis of Onchocerca lupi and its Wolbachia endosymbiont

The morphology of Onchocerca lupi, responsible for canine ocular onchocercosis, is unique within the genus. Earlier analyses of the 5S ribosomal RNA gene spacer region sequence of the parasite and the 16S ribosomal RNA gene sequence of its Wolbachia endosymbiotic bacteria (Rickettsiales) supported t...

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Bibliographic Details
Published in:Veterinary parasitology 2002-09, Vol.108 (2), p.153-161
Main Authors: Egyed, Z, Sréter, T, Széll, Z, Nyirő, G, Márialigeti, K, Varga, I
Format: Article
Language:English
Subjects:
Animals
Bacterial Outer Membrane Proteins - chemistry
Bacterial Outer Membrane Proteins - genetics
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Base Sequence
Co-evolution
Cytoskeletal Proteins
Dog
Dog Diseases - parasitology
Dogs
Electron Transport Complex IV - chemistry
Electron Transport Complex IV - genetics
Molecular Sequence Data
Onchocerca - genetics
Onchocerca - growth & development
Onchocerca lupi
Onchocerciasis, Ocular - parasitology
Onchocerciasis, Ocular - veterinary
Phylogeny
Sequence Alignment
Sequence Analysis, DNA
Symbiosis
Wolbachia - genetics
Wolbachia sp
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Summary:The morphology of Onchocerca lupi, responsible for canine ocular onchocercosis, is unique within the genus. Earlier analyses of the 5S ribosomal RNA gene spacer region sequence of the parasite and the 16S ribosomal RNA gene sequence of its Wolbachia endosymbiotic bacteria (Rickettsiales) supported the morphological and biological arguments that O. lupi is a distinct species. However, the exact phylogenetic position of O. lupi and its endosymbiont could not be unambiguously determined. Herein we report analyses based on the mitochondrial cytochrome oxidase I (COI) gene of the filarial species and the Wolbachia surface protein ( wsp) and the bacterial cell-cycle ftsZ genes of their wolbachiae. Our results indicate that O. lupi separated from other Onchocerca spp. early in evolution. This is in line with the previous morphological analysis demonstrating that O. lupi is an atypical Onchocerca species showing both primitive and evolved characters. The phylogenetic trees generated for the COI sequences of filariae and the wsp and ftsZ sequences of their wolbachiae were congruent with each other, which supports the hypothesis that nematodes and their Wolbachia endobacteria share a long co-evolutionary history.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(02)00186-3