Changes in mitochondrial and microsomal rat liver coenzyme Q9, and Q10 content induced by dietary fat and endogenous lipid peroxidation

• Published in: FEBS letters 1991-08, Vol.287 (1-2), p.89-92
• Main Authors: Huertas, J.R., Battino, M., Lenaz, G., Mataix, F.J.
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Chromatography, High Pressure Liquid; Coenzyme Q; Coenzymes; Coenzymes, vitamins; Corn oil; Corn Oil - administration & dosage; Corn Oil - pharmacology; Dietary Fats, Unsaturated - administration & dosage; Dietary Fats, Unsaturated - pharmacology; Doxorubicin - pharmacology; Fundamental and applied biological sciences. Psychology; HPLC; Lipid Peroxidation; Liver mitochondrion and microsome; Male; Malondialdehyde; Malondialdehyde - metabolism; Microsomes, Liver - metabolism; Mitochondria, Liver - metabolism; Olive Oil; Other biological molecules; Plant Oils - administration & dosage; Plant Oils - pharmacology; Rats; Rats, Inbred Strains; Ubiquinone - metabolism
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(91)80022-U

The influence of different kinds of dietary fat (8%) and of endogenous lipid peroxidation with regard to coenzyme Q9 (CoQ9) and coenzyme Q10 (CoQ10) concentrations in mitochondria and microsomes from rat liver has been investigated by means of an HPLC technique. Although the different diet fats used did not produce any effect on microsomes, it was possible to show that each experimental diet differently influenced the mitochondrial levels of CoQ9 and CoQ10. The highest mitochondrial CoQ content was found in case of a diet supplemented with corn oil. An endogenous oxidative stress induced by adriamycin was able to produce a sharp decrease in mitochondrial CoQ9 levels in the rats to which corn oil was administered. The results suggest that dietary fat ought to be considered when studies concerning CoQ mitochondrial levels are carried out.