A novel, anchorless streptococcal surface protein that binds to human immunoglobulins

We have characterized a novel surface protein from urea extract of whole cells of group A Streptococcus pyogenes (GAS). A major protein band (35 kD) was found to hybridize with human IgG by Western blotting. A search of the N-terminal amino acid sequence of this protein by using the GAS genome seque...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-09, Vol.296 (5), p.1329-1333
Main Authors: Kawabata, Shigetada, Tamura, Yasuharu, Murakami, Jumpei, Terao, Yutaka, Nakagawa, Ichiro, Hamada, Shigeyuki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Outer Membrane Proteins - chemistry
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Binding
Carrier Proteins - chemistry
Carrier Proteins - genetics
Carrier Proteins - metabolism
Consensus Sequence
Genes, Bacterial
Genome
Humans
Immunoglobulin
Immunoglobulin G - metabolism
Immunoglobulins - metabolism
M protein
Molecular Sequence Data
Streptococcus pyogenes
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Summary:We have characterized a novel surface protein from urea extract of whole cells of group A Streptococcus pyogenes (GAS). A major protein band (35 kD) was found to hybridize with human IgG by Western blotting. A search of the N-terminal amino acid sequence of this protein by using the GAS genome sequence database revealed an open reading frame that encoded a 38-kDa protein with a signal peptide sequence. We have named this protein streptococcal immunoglobulin-binding protein 35 (Sib35). It was found to be an anchorless protein with no LPXTG motif, distinct from the M protein superfamily exhibiting immunoglobulin-binding activity, and partially secreted in the culture supernatant. Recombinant Sib35 was also shown to bind human IgA and IgM. The sib35 gene was found in all GAS strains examined, but not in oral, group B, C, or G streptococcal strains. These results suggest that Sib35 is a unique immunoglobulin-binding protein in GAS.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02078-8