Radioiodination of myoglobin with 125I using an on-line solid phase HPLC-coupled procedure

A new procedure is described for the radioiodination of proteins, such as myoglobin, which carry sensitive moieties, such as thiols or porphyrins. Myoglobin has been iodinated with 125I by using the iodinating reagent, IODO-GEN R, which has been adsorbed onto polyol silica and packed in a reactor pr...

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Bibliographic Details
Published in:International journal of radiation applications and instrumentation. Part A, Applied radiation and isotopes Applied radiation and isotopes, 1991, Vol.42 (6), p.585-586
Main Authors: Szabó, Gy, Bulman, R.A., Offenmüller, Katalin
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid
Humans
In Vitro Techniques
Indicators and Reagents
Iodine Radioisotopes
Isotope Labeling - methods
Myoglobin
Urea - analogs & derivatives
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Summary:A new procedure is described for the radioiodination of proteins, such as myoglobin, which carry sensitive moieties, such as thiols or porphyrins. Myoglobin has been iodinated with 125I by using the iodinating reagent, IODO-GEN R, which has been adsorbed onto polyol silica and packed in a reactor pre-column coupled in tandem to a gel permeation chromatography column.
ISSN:0883-2889
DOI:10.1016/0883-2889(91)90167-Y