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Surfactant Protein D Reduces Alveolar Macrophage Apoptosis In Vivo

Surfactant protein D (SP-D) is a molecule of the innate immune system that recognizes the patterns of surface carbohydrate on pathogens and targets them for phagocytosis and killing. SP-D-deficient mice show an increased number of macrophages in the alveolar space, excess surfactant phospholipid, ov...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2002-09, Vol.169 (6), p.2892-2899
Main Authors: Clark, Howard, Palaniyar, Nades, Strong, Peter, Edmondson, Jess, Hawgood, Samuel, Reid, Kenneth B. M
Format: Article
Language:English
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Summary:Surfactant protein D (SP-D) is a molecule of the innate immune system that recognizes the patterns of surface carbohydrate on pathogens and targets them for phagocytosis and killing. SP-D-deficient mice show an increased number of macrophages in the alveolar space, excess surfactant phospholipid, overproduction of reactive oxygen species, and the development of emphysema. We report here that SP-D-deficient mice have a 5- to 10-fold increase in the number of apoptotic and necrotic alveolar macrophages, as defined by annexin V and propidium iodine staining, respectively. Intrapulmonary administration of a truncated 60-kDa fragment of human recombinant SP-D reduces the number of apoptotic and necrotic alveolar macrophages and partially corrects the lipid accumulation in SP-D-deficient mice. The same SP-D fragment binds preferentially to apoptotic and necrotic alveolar macrophages in vitro, suggesting that SP-D contributes to immune homeostasis in the lung by recognizing and promoting removal of necrotic and apoptotic cells.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.169.6.2892