Restriction fragment length polymorphism and polymerase chain reaction: HLA-DQA1 and polymarker analysis of blood samples from transfusion recipients

The effect of blood transfusion on DNA profiles of an individual is a significant issue in the forensic context. In the present study, the effects of blood transfusion in 5 recipients were studied by performing restriction fragment length polymorphism and polymerase chain reaction HLA-DQA1 and Polym...

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Bibliographic Details
Published in:American journal of clinical pathology 2002-09, Vol.118 (3), p.382-387
Main Authors: GIROTI, Rajiv I, BISWAS, Rajesh, MUKHERJEE, Kanchan
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood Transfusion
DNA - blood
DNA - genetics
DNA Fingerprinting
Forensic medicine
Forensic Medicine - methods
Genetic Markers - genetics
HLA-DQ alpha-Chains
HLA-DQ Antigens - analysis
Humans
Male
Medical sciences
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Public health. Hygiene
Public health. Hygiene-occupational medicine
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Summary:The effect of blood transfusion on DNA profiles of an individual is a significant issue in the forensic context. In the present study, the effects of blood transfusion in 5 recipients were studied by performing restriction fragment length polymorphism and polymerase chain reaction HLA-DQA1 and Polymarker (LDLR, GYPA, HBGG, D7S8, and GC) assays (Roche Molecular Systems, Branchburg, NJ) on serial posttransfusion blood samples. Pretransfusion and posttransfusion DNA profiles of all 5 recipients were consistent with no evidence of the donor genetic material. Currently used DNA profiling techniques in forensic science are reliable and informative for paternity and identity purposes in situations involving transfusion of 1 or 2 U of blood up to 24 hours posttransfusion.
ISSN:0002-9173
1943-7722
DOI:10.1309/KAWA-QHBU-6D1J-XY3G