Four-sector tandem mass spectrometric analysis of complex mixtures of phosphatidylcholines present in a human immunodeficiency virus preparation

A number of phosphatidylcholines have been isolated from an HIV-1/MN preparation by reversed-phase high-performance liquid chromatography (HPLC) and analyzed by fast atom bombardment mass spectrometry (FABMS), FABMS/MS, and FABMS/MS/MS in both positive- and negative-ion modes. Negative-ion FABMS/MS...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1991-06, Vol.63 (11), p.1110-1114
Main Authors: Bryant, Duncan K, Orlando, Ronald C, Fenselau, Catherine, Sowder, Raymond C, Henderson, Louis E
Format: Article
Language:English
Subjects:
AIDS/HIV
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
HIV-1 - analysis
Humans
Mass Spectrometry
Microbiology
Miscellaneous
Phosphatidylcholines - analysis
Virology
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Summary:A number of phosphatidylcholines have been isolated from an HIV-1/MN preparation by reversed-phase high-performance liquid chromatography (HPLC) and analyzed by fast atom bombardment mass spectrometry (FABMS), FABMS/MS, and FABMS/MS/MS in both positive- and negative-ion modes. Negative-ion FABMS/MS with high-energy collisions was used to identify the length of the acyl groups and the degree of saturation, as well as their position on the glyceride group. FABMS/MS in the positive-ion mode was used to identify the polar head group. Negative-ion FABMS/MS/MS was used to locate positions of double bonds in acyl groups. We find that four-sector tandem mass spectrometry with high-energy collisional activation provides qualitative analysis of viral phosphatidyl lipids in considerable detail, as well as semiquantitative information. Approximate quantitation of the phosphatidylcholine content of the HIV-1/MN preparation by measuring relative peak heights of molecular ions in FABMS reveals an array of phosphatidylcholines consistent with that found in human erythrocytes, indicating the likely source of lipids in the viral membrane to be the host cell membrane.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00011a010