Determination of Cobalt in Urine by FI-ICP-AES with Online Preconcentration

A method for the preconcentration and determination of cobalt in human urine samples was developed. The online preconcentration and determination were attained using inductively coupled plasma atomic emission spectromety (ICP-AES) coupled to a flow injection (FI) method. Cobalt was retained on an Am...

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Bibliographic Details
Published in:Journal of analytical toxicology 2002-09, Vol.26 (6), p.360-364
Main Authors: Farias, Gustavo M., Wuilloud, Rodolfo G., Moyano, Susana, Gásquez, José A., Olsina, Roberto A., Martinez, Luis D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calibration
Chemical and industrial products toxicology. Toxic occupational diseases
Cobalt - urine
Humans
Medical sciences
Metals and various inorganic compounds
Sensitivity and Specificity
Specimen Handling
Spectrum Analysis - methods
Toxicology
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Summary:A method for the preconcentration and determination of cobalt in human urine samples was developed. The online preconcentration and determination were attained using inductively coupled plasma atomic emission spectromety (ICP-AES) coupled to a flow injection (FI) method. Cobalt was retained on an Amberlite XAD-7 resin as cobalt-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol complex at pH 9.5. Cobalt was removed from the microcolumn with perchloric acid. A sensitivity enhancement factor of 90 was obtained with respect to cobalt determination by ICP-AES without preconcentration. The value of detection limit for the preconcentration method proposed was 25 ng/L. The precision for 10 replicate determinations at the 5 mg/L (mean ± SD, 5.1 ± 0.14) Co level was 2.7% relative standard deviation, calculated from the peak heights obtained. The calibration graph preconcentration method for cobalt was linear with a correlation coefficient of 0.9994 from approximately 0.25 mg/L up to at least 100 mg/L. The method was successfully applied to the determination of cobalt in human urine samples.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/26.6.360