Sp3 Expression in Immune Cells: A Quantitative Study

We previously reported the lack of expression of the bifunctional transcription factor Sp3 in peripheral blood mononuclear cells from most patients with multiple sclerosis (MS) (Grekova et al, 1996). An RT-PCR technique was developed to evaluate Sp3 mRNA levels in peripheral blood mononuclear cell s...

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Bibliographic Details
Published in:Laboratory investigation 2002-09, Vol.82 (9), p.1131-1138
Main Authors: Grekova, Maria C, Salerno, Kilian, Mikkilineni, Radha, Richert, John R
Format: Article
Language:English
Subjects:
Actins - genetics
Base Sequence
Biological and medical sciences
DNA-Binding Proteins - genetics
Humans
Laboratory Medicine
Leukocytes, Mononuclear - metabolism
Medical sciences
Medicine
Medicine & Public Health
Molecular Sequence Data
Multiple Sclerosis - metabolism
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
Neurology
Pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Sp3 Transcription Factor
Transcription Factors - genetics
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Summary:We previously reported the lack of expression of the bifunctional transcription factor Sp3 in peripheral blood mononuclear cells from most patients with multiple sclerosis (MS) (Grekova et al, 1996). An RT-PCR technique was developed to evaluate Sp3 mRNA levels in peripheral blood mononuclear cell subsets. Semi-quantitative and quantitative competitive RT-PCR assays were used to compare the level of Sp3 expression among subjects and among immune cell subsets. The competitor DNA fragment contained a deletion from the normal Sp3 cDNA sequence. The wild-type Sp3 cDNA and the competitor DNA fragment amplified with equal efficiency, and the two PCR products were distinguished by size. These studies demonstrated that normal CD4+ and CD8+ T cells, B cells, and macrophages expressed comparable amounts of Sp3 mRNA. No Sp3 expression could be detected in normal natural killer cells nor in any of these cell types from Sp3-negative MS patients. We propose that transcription of the Sp3 gene is blocked in immune cells from most patients with MS and that this contributes to the development of central nervous system inflammation in the disease.
ISSN:0023-6837
1530-0307
DOI:10.1097/01.LAB.0000029149.38881.84