Optimizing lipases and related enzymes for efficient application

• Published in: Trends in Biotechnology 2002-10, Vol.20 (10), p.433-437
• Main Authors: Bornscheuer, Uwe T., Bessler, Cornelius, Srinivas, Ramisetti, Hari Krishna, Sajja
• Format: Article
• Language: English
• Subjects: Bacteria - enzymology; Bacteria - genetics; Biological and medical sciences; Biotechnology; Databases, Protein; Directed evolution; Directed Molecular Evolution - methods; Enzyme Stability; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - classification; Enzymes, Immobilized - genetics; Enzymes, Immobilized - metabolism; Esterase; Fundamental and applied biological sciences. Psychology; Fungi - enzymology; Fungi - genetics; Immobilization of enzymes and other molecules; Immobilization techniques; Lipase; Lipase - chemistry; Lipase - classification; Lipase - genetics; Lipase - metabolism; Methods. Procedures. Technologies; Non-aqueous media; Organic solvents; Protein design; Protein Engineering - methods; Species Specificity
• ISSN: 0167-7799; 1879-3096
• DOI: 10.1016/S0167-7799(02)02046-2

Although numerous reactions have been performed using lipases and related enzymes (e.g. esterases and phospholipases), it is still a challenge to identify the most suitable biocatalyst and best reaction conditions for an efficient application. Frequently used methods such as immobilization and optimization of the reaction medium cannot be transferred from one reaction system or substrate to another. However, in the past few years, rational protein design and directed evolution have emerged as efficient alternative methods to optimize biocatalytic reactions. In the past few years, rational protein design and directed evolution have emerged as efficient alternative methods to optimize biocatalytic reactions.