Quantitation of calcitonin gene-related peptide mrna and neuronal cell death in facial motor nuclei following axotomy and 633 nm low power laser treatment

• Published in: Lasers in surgery and medicine 2002-09, Vol.31 (3), p.216-222
• Main Authors: Snyder, Sara K., Byrnes, Kimberly R., Borke, Rosemary C., Sanchez, Ana, Anders, Juanita J.
• Format: Article
• Language: English
• Subjects: 633 nm; Animals; Axotomy; Biological and medical sciences; biostimulation; Calcitonin Gene-Related Peptide - immunology; Calcitonin Gene-Related Peptide - metabolism; CGRP; Diseases of the nervous system; facial nerve transection; Female; light therapy; LLLT; low level laser therapy; Low-Level Light Therapy; Medical sciences; Motor Neurons - metabolism; Nerve Regeneration - physiology; PCR quantitation; Peripheral Nerve Injuries; Peripheral Nerves - physiology; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Up-Regulation - radiation effects
• ISSN: 0196-8092; 1096-9101
• DOI: 10.1002/lsm.10098

Background and Objectives A persistent increase in calcitonin gene‐related peptide (CGRP) immunoreactivity in motoneurons may serve as an indicator for regeneration after peripheral nerve injury [Borke et al., J Neurocytol 1993;22:141–153]. Study Design/Materials and Methods We examined the effects of low power laser treatment (633 nm) on axotomy‐induced changes in α‐CGRP mRNA and long‐term neuronal survival in facial motoneurons. A quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) assay for α‐CGRP mRNA was used to detect changes in the response to axotomy and laser irradiation. Cell counts of neurons in injured and non‐injured facial motor nuclei of laser‐treated and non‐treated rats were done to estimate neuronal survival. Results A 10‐fold increase (P