γ Heavy chain disease screening showing a discrepancy between electrophoretic and nephelometric determinations of serum γ globulin concentration

A 75-year-old woman with rheumatoid arthritis showed a discrepancy between the reduced level of serum γ globulin on cellulose acetate electrophoresis and the normal level of serum IgG determined by laser nephelometry. Although no M-peak was detectable on cellulose acetate electrophoresis, immunoelec...

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Bibliographic Details
Published in:Annals of clinical biochemistry 2002-09, Vol.39 (5), p.531-533
Main Authors: TAKATANI, Tsunenori, MORITA, Keiko, TAKAOKA, Naomi, TATSUMI, Masatsuga, OKUNO, Yorio, MASUTANI, Takayuki, MURAKAWA, Koichi, FUKUI, Akihiro, TSUKAGUCHI, Nobuhiko, OKAMOTO, Yasuyuki
Format: Article
Language:English
Subjects:
Aged
Biological and medical sciences
Blotting, Western
Diagnostic Errors
Electrophoresis, Cellulose Acetate
Female
gamma-Globulins - analysis
Heavy Chain Disease - blood
Heavy Chain Disease - diagnosis
Humans
Immunodeficiencies. Immunoglobulinopathies
Immunoelectrophoresis
Immunoglobulin G - blood
Immunoglobulinopathies
Immunopathology
Mass Screening
Medical sciences
Nephelometry and Turbidimetry
Statistics as Topic
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Summary:A 75-year-old woman with rheumatoid arthritis showed a discrepancy between the reduced level of serum γ globulin on cellulose acetate electrophoresis and the normal level of serum IgG determined by laser nephelometry. Although no M-peak was detectable on cellulose acetate electrophoresis, immunoelectrophoresis of the patient's serum revealed a monoclonal protein reacting with anti-IgG antiserum but not with anti-κ or anti-λ light chain antiserum. Western blotting of the patient's serum showed abnormal low-molecular-weight γ chains. Thus, the patient was diagnosed with γ heavy chain disease. A comparison of γ globulin levels determined by different methods may be useful when screening for this disease.
ISSN:0004-5632
1758-1001
DOI:10.1258/000456302320314601