Identification of epidemic strains of Streptococcus suis by genomic fingerprinting

A natural outbreak of Streptococcus suis meningitis in two closed swine herds was studied. DNA fingerprinting, serotyping, and biochemical profiles were assessed. Multiple serotypes were recovered from these herds. In farm A, 50 S. suis strains were isolated from 330 swabs collected. Eighteen strain...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1991-04, Vol.29 (4), p.782-787
Main Authors: Mogollon, J.D. (University of Minnesota, Minneapolis, MN), Pijoan, C, Murtaugh, M.P, Collins, J.E, Cleary, P.P
Format: Article
Language:English
Subjects:
ADN
Animals
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
CERDO
CLONE
CLONES
Disease Outbreaks
DNA Fingerprinting
EPIDEMIOLOGIA
EPIDEMIOLOGIE
Fundamental and applied biological sciences. Psychology
GENETICA
GENETIQUE
LECHON
MENINGITE
MENINGITIS
Meningitis - epidemiology
Meningitis - pathology
Meningitis - veterinary
Microbiology
MINNESOTA
Minnesota - epidemiology
PODER PATOGENO
POLIMORFISMO
POLYMORPHISME
PORCELET
PORCIN
Postmortem Changes
POUVOIR PATHOGENE
Restriction Mapping
SEROTIPOS
SEROTYPE
Streptococcal Infections - epidemiology
Streptococcal Infections - pathology
Streptococcal Infections - veterinary
Streptococcus - isolation & purification
STREPTOCOCCUS SUIS
Swine
Swine Diseases - epidemiology
Swine Diseases - pathology
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
TRANSMISION DE ENFERMEDADES
TRANSMISSION DES MALADIES
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Summary:A natural outbreak of Streptococcus suis meningitis in two closed swine herds was studied. DNA fingerprinting, serotyping, and biochemical profiles were assessed. Multiple serotypes were recovered from these herds. In farm A, 50 S. suis strains were isolated from 330 swabs collected. Eighteen strains belonged to serotype 2, and 32 strains belonged to serotypes 3, 5, 6, 8, 9, and 11. In farm B, 16 S. suis strains were recovered from a total of 70 samples. Eight strains belonged to serotype 7, and eight belonged to serotypes 2, 3, 5, and 8. In each epidemiological situation, a single strain characterized by a distinctive restriction fragment pattern predominated among affected penmates. The epidemic serotype 2 strain was detected in farm A in weaned pigs between the ages of 5 and 7 weeks. In contrast, the pathogenic strain in farm B belonged to serotype 7 and was isolated from pigs up to 3 weeks of age. The results from both farms strongly suggest a lateral spread of these organisms. No vertical transmission could be shown in either herd. It was concluded that genomic fingerprinting is an appropriate method to distinguish outbreak isolates of S. suis from nonoutbreak strains, within the same serotype or from epidemiologically unrelated clusters of strains
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.29.4.782-787.1991