Comparison of EST libraries from seven beetle species: towards a framework for phylogenomics of the Coleoptera

Relatively little is known about Coleoptera genes and genomes and how these compare in different taxa. We describe here the construction, DNA sequencing and sequence comparisons of cDNA libraries from seven beetle species. A total of 6717 bacterial colonies were screened for cDNA insert containing p...

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Bibliographic Details
Published in:Insect molecular biology 2002-10, Vol.11 (5), p.467-475
Main Authors: Theodorides, K., De Riva, A., Gómez-Zurita, J., Foster, P. G., Vogler, A. P.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Coleoptera
Coleoptera - classification
Coleoptera - genetics
Contig Mapping
Databases, Nucleic Acid
DNA, Complementary
Expressed Sequence Tags
Gene Library
Genes, Insect
genome
Molecular Sequence Data
Phylogeny
transcriptome
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Summary:Relatively little is known about Coleoptera genes and genomes and how these compare in different taxa. We describe here the construction, DNA sequencing and sequence comparisons of cDNA libraries from seven beetle species. A total of 6717 bacterial colonies were screened for cDNA insert containing plasmids and 2784 size selected clones were 5′‐ and 3′‐end sequenced to produce 1620 assembled sequences. Similarity comparisons with existing protein sequence databases revealed that 65.1% had matches (E 
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.2002.00355.x