Drosophila yolk protein produced in E. coli is accumulated by mosquito ovaries

Despite similar functions, the yolk proteins of the higher dipteran flies and the vitellogenins found in other insects are unrelated at the sequence level and have evolved from different genes. Both are selectively endocytosed into the ovary via receptors belonging to the LDLR receptor subfamily. We...

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Published in:Insect molecular biology 2002-10, Vol.11 (5), p.487-496
Main Authors: Bownes, M., Hurd, H., Büsgen, T., Servay, D., Alvis, S., Popovic, B., Bruce, S., Burns, I., Rothwell, K., Walkinshaw, Malcolm
Format: Article
Language:English
Subjects:
Animals
Anopheles - metabolism
Cloning, Molecular
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
endocytosis
Escherichia coli
evolution
Female
Gene Expression
Genetic Vectors
Ovary - metabolism
reproduction
vitellogenesis
Vitellogenins - genetics
Vitellogenins - metabolism
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container_end_page 496
container_issue 5
container_start_page 487
container_title Insect molecular biology
container_volume 11
creator Bownes, M.
Hurd, H.
Büsgen, T.
Servay, D.
Alvis, S.
Popovic, B.
Bruce, S.
Burns, I.
Rothwell, K.
Walkinshaw, Malcolm
description Despite similar functions, the yolk proteins of the higher dipteran flies and the vitellogenins found in other insects are unrelated at the sequence level and have evolved from different genes. Both are selectively endocytosed into the ovary via receptors belonging to the LDLR receptor subfamily. We cloned the Drosophila yp1 gene into an E. coli expression vector and showed that the yolk protein produced by E. coli is taken up into ovaries of both Drosophila melanogaster and the malaria mosquito Anopheles gambiae, which normally uses vitellogenin.
doi_str_mv 10.1046/j.1365-2583.2002.00357.x
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subjects Animals
Anopheles - metabolism
Cloning, Molecular
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
endocytosis
Escherichia coli
evolution
Female
Gene Expression
Genetic Vectors
Ovary - metabolism
reproduction
vitellogenesis
Vitellogenins - genetics
Vitellogenins - metabolism
title Drosophila yolk protein produced in E. coli is accumulated by mosquito ovaries
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