DNA Damage and Micronucleus Induction in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Continuous-Wave Radiofrequency Field

• Published in: Radiation research 2002-10, Vol.158 (4), p.523-533
• Main Authors: McNamee, J. P., Bellier, P. V., Gajda, G. B., Miller, S. M., Lemay, E. P., Lavallée, B. F., Marro, L., Thansandote, A.
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Blood; Blood cells; Cells, Cultured; Comets; Coolants; Cultured cells; DNA Damage; Female; Humans; Incubators; Leukocytes; Leukocytes - diagnostic imaging; Leukocytes - radiation effects; Male; Medical sciences; Micronuclei, Chromosome-Defective - radiation effects; Middle Aged; Radiation damage; Radio Waves; REGULAR ARTICLES; Space life sciences; Ultrasonography; Waveguides
• ISSN: 0033-7587; 1938-5404
• DOI: 10.1667/0033-7587(2002)158[0523:DDAMII]2.0.CO;2

McNamee, J. P., Bellier, P. V., Gajda, G. B., Miller, S. M., Lemay E. P., Lavallée, B. F., Marro, L. and Thansandote, A. DNA Damage and Micronucleus Induction in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Continuous-Wave Radiofrequency Field. Radiat. Res. 158, 523–533 (2002). Human blood cultures were exposed to a 1.9 GHz continuous-wave (CW) radiofrequency (RF) field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) of 0.0, 0.1, 0.26, 0.92, 2.4 and 10 W/kg were achieved, and the temperature within the cultures during a 2-h exposure was maintained at 37.0 ± 0.5°C. Concurrent negative (incubator) and positive (1.5 Gy 137Cs γ radiation) control cultures were run for each experiment. DNA damage was quantified immediately after RF-field exposure using the alkaline comet assay, and four parameters (tail ratio, tail moment, comet length and tail length) were used to assess DNA damage for each comet. No evidence of increased primary DNA damage was detected by any parameter for RF-field-exposed cultures at any SAR tested. The formation of micronuclei in the RF-field-exposed blood cell cultures was assessed using the cytokinesis-block micronucleus assay. There was no significant difference in the binucleated cell frequency, incidence of micronucleated binucleated cells, or total incidence of micronuclei between any of the RF-field-exposed cultures and the sham-exposed controls at any SAR tested. These results do not support the hypothesis that acute, nonthermalizing 1.9 GHz CW RF-field exposure causes DNA damage in cultured human leukocytes.