Establishment of Immortal Normal and Ataxia Telangiectasia Fibroblast Cell Lines by Introduction of the hTERT Gene

To establish immortal human cells, we introduced the human catalytic subunit of telomerase(hTERT) gene into skin fibroblast cells obtained from normal and ataxia telangiectasia(AT) individuals of Japanese origin. After hTERT introduction, these cells continue to grow beyond a population doubling num...

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Bibliographic Details
Published in:JOURNAL OF RADIATION RESEARCH 2002, Vol.43 (2), p.167-174
Main Authors: Nakamura, Hideaki, Fukami, Hiroko, Hayashi, Yuko, Kiyono, Tohru, Nakatsugawa, Shigekazu, Hamaguchi, Michinari, Ishizaki, Kanji
Format: Article
Language:English
Subjects:
Analysis
Ataxia
Ataxia telangiectasia
Ataxia Telangiectasia - genetics
Ataxia Telangiectasia - pathology
Cell Line, Transformed
Cells (Biology)
DNA-Binding Proteins
Fibroblasts - pathology
Gene Transfer Techniques
Genes
Humans
Reference Values
Telomerase
Telomerase - genetics
Telomeres
Tumor proteins
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Summary:To establish immortal human cells, we introduced the human catalytic subunit of telomerase(hTERT) gene into skin fibroblast cells obtained from normal and ataxia telangiectasia(AT) individuals of Japanese origin. After hTERT introduction, these cells continue to grow beyond a population doubling number of 200 while maintaining their original radiosensitivity. Inductions of p53, phosphorylation of Ser15 in p53, and induction of p21 by X-ray irradiation in immortal cells derived from normal individual were not affected by the hTERT introduction. Both normal and AT immortal cells exhibited an apparent inhibition of growth as original primary cells when they reached confluence. Karyotype analysis has revealed that they are in a diploid range. These results suggest that cells immortalized by hTERT introduction retain their original characteristics except for immortalization, and that they may be useful for analyzing various effects of radiation on human cells.
ISSN:0449-3060
1349-9157
DOI:10.1269/jrr.43.167