Pathogenic effects of bacteria isolated from larvae of Hylesia metabus Crammer (Lepidoptera: Saturniidae)

• Published in: Journal of invertebrate pathology 2002-05, Vol.80 (1), p.7-12
• Main Authors: Osborn, Frances, Berlioz, Liliana, Vitelli-Flores, Juana, Monsalve, Wilmer, Dorta, Blas, Rodrı́guez Lemoine, Vidal
• Format: Article
• Language: English
• Subjects: Animals; Bacteria - classification; Bacteria - isolation & purification; Bacteria - pathogenicity; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Hylesia metabus; Larva - microbiology; Larvae; Microbiology; Moths - microbiology; Pathogenic effects; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Pseudomonas aeruginosa
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/S0022-2011(02)00037-X

Hylesia metabus larvae are susceptible to several pathogens indigenous to the area in which they are found. Some larvae show symptoms characteristic of bacterial infection; they become flaccid and lethargic, and show a marked loss of appetite. We isolated and identified 29 bacterial strains from live, dead and experimentally infected H. metabus larvae, and evaluated their pathogenic activity. The bacteria which caused mortality in the larvae were: Pseudomonas aeruginosa (60–93.3%), Proteus vulgaris (20%), Alcaligenes faecalis, Planococcus sp. and Bacillus megaterium (10%), at doses of 3–4×10 7. Although P. aeruginosa is a well-known insect pathogen, this is the first report of its pathogenic activity on H. metabus. The potential risk to humans and low virulence make it unlikely that P. aeruginosa could be used in an augmentative biological control programme. However its natural incidence may be enhanced using parasites and predators of H. metabus as carriers.