Carbon fiber microelectrodes with multiple sensing elements for in vivo voltammetry

Electrically evoked dopamine release was monitored in the striatum of anesthetized rats using voltammetric microelectrode assemblies with two to four separately addressable carbon fiber sensing elements. The sensing elements were disk-shaped, had a diameter of about 1 μm, and were separated from eac...

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Bibliographic Details
Published in:Journal of neuroscience methods 2002-09, Vol.119 (1), p.75-81
Main Authors: Dressman, Shawn F, Peters, Jennifer L, Michael, Adrian C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carbon
Carbon Fiber
Corpus Striatum - physiology
Dopamine
Dopamine - metabolism
Electric Stimulation
Electrical stimulation
Electrochemistry - instrumentation
Extracellular Space - metabolism
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
Male
Medial forebrain bundle
Medial Forebrain Bundle - physiology
Microelectrode
Microelectrodes
Microscopy, Electron, Scanning
Rats
Rats, Sprague-Dawley
Striatum
Vertebrates: nervous system and sense organs
Voltammetry
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Summary:Electrically evoked dopamine release was monitored in the striatum of anesthetized rats using voltammetric microelectrode assemblies with two to four separately addressable carbon fiber sensing elements. The sensing elements were disk-shaped, had a diameter of about 1 μm, and were separated from each other by less than 15 μm. The microelectrodes were used to monitor extracellular dopamine at multiple depths beneath the brain surface during electrical stimulation of the medial forebrain bundle. When the sensing elements were 10–15 μm apart, the stimulus responses at each element were distinct, suggesting that each response was representative of a distinct recording site. The possibility of performing measurements at distinct but closely spaced sites provides a potential route to high spatial resolution information about extracellular events. On the other hand, when the individual sensing elements were about 1 μm apart, similarities between the observed stimulus responses suggest that the multiple elements were recording from a single site. The ability to perform multiple chemical measurements at a single site presents several opportunities for new approaches to the in vivo study of neurochemistry.
ISSN:0165-0270
1872-678X
DOI:10.1016/S0165-0270(02)00180-2