Expression of angiogenic and neurotrophic factors in the human amnion and choriodecidua

Objective: Our objective was to identify the novel or differential expression of growth or development associated genes in the human gestational membranes that might play roles in pregnancy or in term or preterm parturition. Study Design: Complementary DNA arrays were probed with [α33P]dCTP-labeled-...

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Published in:American journal of obstetrics and gynecology 2002-09, Vol.187 (3), p.728-734
Main Authors: Marvin, Keith W., Keelan, Jeffrey A., Eykholt, Roberta L., Sato, Timothy A., Mitchell, Murray D.
Format: Article
Language:English
Subjects:
Amnion
Amnion - metabolism
Angiogenesis Inducing Agents - genetics
Angiogenesis Inducing Agents - physiology
Biological and medical sciences
choriodecidua
Chorion - metabolism
cytokine
Decidua - metabolism
Diseases of mother, fetus and pregnancy
expression array
Female
Gene Expression Profiling
gestational tissue
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Nerve Growth Factors - genetics
Nerve Growth Factors - physiology
Pregnancy
Pregnancy. Fetus. Placenta
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Summary:Objective: Our objective was to identify the novel or differential expression of growth or development associated genes in the human gestational membranes that might play roles in pregnancy or in term or preterm parturition. Study Design: Complementary DNA arrays were probed with [α33P]dCTP-labeled-complementary DNA that was prepared from the RNA of reflected amnion and choriodecidua that represent term not-in-labor, term spontaneous labor, and preterm labor with and without chorioamnionitis (n = 4 per group). Differential expression (term not-in-labor vs term spontaneous labor or preterm labor with chorioamnionitis vs preterm labor without chorioamnionitis) was evaluated by Wilcoxon tests. Results: All 16 amnion samples expressed angiogenic factors (endothelin-2 and -3, vascular endothelial growth factor, and vascular endothelial growth factor–B) and neurotrophic factors (ephrin-A2, ephrin receptors-A2, -B1, -B3, -B4, and -B5, neuropilin-2, p75/nerve growth factor receptor and semaphorin-F). In both amnion and choriodecidua, the expression of vascular endothelial growth factor and the angiopoietin receptor, Tie-2, were greater with term spontaneous labor than with term not-in-labor (P
ISSN:0002-9378
1097-6868
DOI:10.1067/mob.2002.126286