Transport of cis- and trans-4-[ 18F]fluoro-L-proline in F98 glioma cells

• Published in: Nuclear medicine and biology 2002-08, Vol.29 (6), p.685-692
• Main Authors: Langen, Karl-J, Mühlensiepen, Heinz, Schmieder, Sven, Hamacher, Kurt, Bröer, Stefan, Börner, Anne R, Schneeweiss, Frank H.A, Coenen, Heinz H
• Format: Article
• Language: English
• Subjects: Abdomen - diagnostic imaging; Adult; Amino acid transport; Animals; Back - diagnostic imaging; Biological and medical sciences; Biological Transport, Active; Cis- and trans-4-[ 18F]fluoro-L-proline; Female; Glioma - diagnostic imaging; Glioma - metabolism; Glioma cells; Humans; Medical sciences; PET; Proline - analogs & derivatives; Proline - pharmacokinetics; Radiopharmaceuticals; Rats; Reference Values; Sensitivity and Specificity; Thorax - diagnostic imaging; Thorax - metabolism; Tissue Distribution; Tomography, Emission-Computed; Tumor Cells, Cultured; Whole body distribution; Whole-Body Counting
• ISSN: 0969-8051; 1872-9614
• DOI: 10.1016/S0969-8051(02)00327-X

The transport mechanisms of cis-4-[ 18F]fluoro-L-proline (cis-FPro) and trans-4-[ 18F]fluoro-L-proline (trans-FPro) were studied in F98 rat glioma cells in comparison to the natural parent [ 3H]-L-proline. Uptake rates of cis-FPro and trans-FPro in F98 glioma cells were 50–70% lower than those of [ 3H]-L-proline. The amino transport system A inhibitor MeAIB reduced the uptake of [ 3H]-L-proline by 30% and uptake of cis-FPro by 46% while uptake of trans-FPro was not significantly changed. BCH inhibited the uptake of all tracers by 35–44%, serine by 70–90% and L-proline by 60 –80%. Absence of Na + reduced uptake of all tracers significantly but no further inhibitory effect could be observed which suggests a component of unspecific uptake. Radioactivity of cis- and trans-FPro in the acid precipitable fraction was