Expression and regulation of Q8b in a transfected cell line

RNase protection experiments showed that Q8b was actively transcribed in a stably transfected cell line. Moreover, Q8b responded to interferon-gamma (IFN-gamma) treatment with increased levels of mRNA expression. Thus Q8b demonstrates a regulatory response to IFN-gamma characteristic of many other c...

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Bibliographic Details
Published in:Immunogenetics (New York) 1991, Vol.34 (3), p.179-184
Main Authors: WATERS, J. B, FLAHERTY, L
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface - analysis
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes, MHC Class I
Interferon-gamma - pharmacology
Mice
Molecular and cellular biology
Molecular genetics
Phosphatidylinositols - metabolism
RNA, Messenger - analysis
Transfection
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Summary:RNase protection experiments showed that Q8b was actively transcribed in a stably transfected cell line. Moreover, Q8b responded to interferon-gamma (IFN-gamma) treatment with increased levels of mRNA expression. Thus Q8b demonstrates a regulatory response to IFN-gamma characteristic of many other class I genes. Cell surface expression of a Q8b product could also be detected by flow cytometric analysis with the Qa-2-specific monoclonal antibody D3.262. The expression of the Q8b cell surface product increased only slightly after cells were treated with IFN-gamma. The Q8b cell surface product was not sensitive to cleavage by phosphatidylinositol-phospholipase C. These results suggest that the Q8b product, unlike the predominant forms of Qa-2-bearing molecules, is not anchored via phosphatidylinositol to the cell membrane. These results also suggest that Q8b has the potential to contribute to the Qa-2 phenotype in vivo.
ISSN:0093-7711
1432-1211