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Expression and regulation of Q8b in a transfected cell line

RNase protection experiments showed that Q8b was actively transcribed in a stably transfected cell line. Moreover, Q8b responded to interferon-gamma (IFN-gamma) treatment with increased levels of mRNA expression. Thus Q8b demonstrates a regulatory response to IFN-gamma characteristic of many other c...

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Published in:	Immunogenetics (New York) 1991, Vol.34 (3), p.179-184
Main Authors:	WATERS, J. B, FLAHERTY, L
Format:	Article
Language:	English
Subjects:	Animals Antigens, Surface - analysis Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes, MHC Class I Interferon-gamma - pharmacology Mice Molecular and cellular biology Molecular genetics Phosphatidylinositols - metabolism RNA, Messenger - analysis Transfection
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container_title	Immunogenetics (New York)
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creator	WATERS, J. B FLAHERTY, L
description	RNase protection experiments showed that Q8b was actively transcribed in a stably transfected cell line. Moreover, Q8b responded to interferon-gamma (IFN-gamma) treatment with increased levels of mRNA expression. Thus Q8b demonstrates a regulatory response to IFN-gamma characteristic of many other class I genes. Cell surface expression of a Q8b product could also be detected by flow cytometric analysis with the Qa-2-specific monoclonal antibody D3.262. The expression of the Q8b cell surface product increased only slightly after cells were treated with IFN-gamma. The Q8b cell surface product was not sensitive to cleavage by phosphatidylinositol-phospholipase C. These results suggest that the Q8b product, unlike the predominant forms of Qa-2-bearing molecules, is not anchored via phosphatidylinositol to the cell membrane. These results also suggest that Q8b has the potential to contribute to the Qa-2 phenotype in vivo.
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The expression of the Q8b cell surface product increased only slightly after cells were treated with IFN-gamma. The Q8b cell surface product was not sensitive to cleavage by phosphatidylinositol-phospholipase C. These results suggest that the Q8b product, unlike the predominant forms of Qa-2-bearing molecules, is not anchored via phosphatidylinositol to the cell membrane. These results also suggest that Q8b has the potential to contribute to the Qa-2 phenotype in vivo.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>1654299</pmid><tpages>6</tpages></addata></record>
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subjects	Animals Antigens, Surface - analysis Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes, MHC Class I Interferon-gamma - pharmacology Mice Molecular and cellular biology Molecular genetics Phosphatidylinositols - metabolism RNA, Messenger - analysis Transfection
title	Expression and regulation of Q8b in a transfected cell line
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