The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay

Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. s...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2002-10, Vol.82 (2), p.239-242
Main Authors: Siti Najila, M.J, Noor Rain, A, Mohamad Kamel, A.G, Syed Zahir, S.I, Khozirah, S, Lokman Hakim, S, Zakiah, I, Azizol, A.K
Format: Article
Language:English
Subjects:
Aldehyde Oxidoreductases - metabolism
Andrographis
Animals
Annonaceae
Antimalarials - isolation & purification
Antimalarials - pharmacology
Antimalarials - therapeutic use
Biological and medical sciences
Biological Assay - methods
Biological Assay - statistics & numerical data
Drug Evaluation, Preclinical - methods
General pharmacology
Humans
In vitro anti-malarial activity
Lactate dehydrogenase assay
Medical sciences
Medicinal plants
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Plant Leaves
Plant Stems
Plasmodium falciparum
Plasmodium falciparum - drug effects
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Summary:Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. Furthermore a better IC 50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC 50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.
ISSN:0378-8741
1872-7573
DOI:10.1016/S0378-8741(02)00183-6