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The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay
Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. s...
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| Published in: | Journal of ethnopharmacology 2002-10, Vol.82 (2), p.239-242 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
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| cited_by | cdi_FETCH-LOGICAL-c391t-f762711fafe9783de46df28f7068ff47c2ce298d06c3b123dd4d9459c7b6aa13 |
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| cites | cdi_FETCH-LOGICAL-c391t-f762711fafe9783de46df28f7068ff47c2ce298d06c3b123dd4d9459c7b6aa13 |
| container_end_page | 242 |
| container_issue | 2 |
| container_start_page | 239 |
| container_title | Journal of ethnopharmacology |
| container_volume | 82 |
| creator | Siti Najila, M.J Noor Rain, A Mohamad Kamel, A.G Syed Zahir, S.I Khozirah, S Lokman Hakim, S Zakiah, I Azizol, A.K |
| description | Goniothalamus scortechinii,
Andrographis paniculata and
Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards
Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of
G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of
Plasmodium falciparum. Furthermore a better IC
50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC
50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10. |
| doi_str_mv | 10.1016/S0378-8741(02)00183-6 |
| format | article |
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Andrographis paniculata and
Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards
Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of
G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of
Plasmodium falciparum. Furthermore a better IC
50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC
50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/S0378-8741(02)00183-6</identifier><identifier>PMID: 12242001</identifier><identifier>CODEN: JOETD7</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Aldehyde Oxidoreductases - metabolism ; Andrographis ; Animals ; Annonaceae ; Antimalarials - isolation & purification ; Antimalarials - pharmacology ; Antimalarials - therapeutic use ; Biological and medical sciences ; Biological Assay - methods ; Biological Assay - statistics & numerical data ; Drug Evaluation, Preclinical - methods ; General pharmacology ; Humans ; In vitro anti-malarial activity ; Lactate dehydrogenase assay ; Medical sciences ; Medicinal plants ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; Plant Leaves ; Plant Stems ; Plasmodium falciparum ; Plasmodium falciparum - drug effects</subject><ispartof>Journal of ethnopharmacology, 2002-10, Vol.82 (2), p.239-242</ispartof><rights>2002 Elsevier Science Ireland Ltd</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-f762711fafe9783de46df28f7068ff47c2ce298d06c3b123dd4d9459c7b6aa13</citedby><cites>FETCH-LOGICAL-c391t-f762711fafe9783de46df28f7068ff47c2ce298d06c3b123dd4d9459c7b6aa13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13906330$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12242001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Siti Najila, M.J</creatorcontrib><creatorcontrib>Noor Rain, A</creatorcontrib><creatorcontrib>Mohamad Kamel, A.G</creatorcontrib><creatorcontrib>Syed Zahir, S.I</creatorcontrib><creatorcontrib>Khozirah, S</creatorcontrib><creatorcontrib>Lokman Hakim, S</creatorcontrib><creatorcontrib>Zakiah, I</creatorcontrib><creatorcontrib>Azizol, A.K</creatorcontrib><title>The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Goniothalamus scortechinii,
Andrographis paniculata and
Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards
Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of
G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of
Plasmodium falciparum. Furthermore a better IC
50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC
50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.</description><subject>Aldehyde Oxidoreductases - metabolism</subject><subject>Andrographis</subject><subject>Animals</subject><subject>Annonaceae</subject><subject>Antimalarials - isolation & purification</subject><subject>Antimalarials - pharmacology</subject><subject>Antimalarials - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - methods</subject><subject>Biological Assay - statistics & numerical data</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>In vitro anti-malarial activity</subject><subject>Lactate dehydrogenase assay</subject><subject>Medical sciences</subject><subject>Medicinal plants</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - therapeutic use</subject><subject>Plant Leaves</subject><subject>Plant Stems</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - drug effects</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkc2O0zAUhSMEYsrAI4C8AYFEwD9pnKxQNYIBaSQWdG_d2tfTixKn2M6IvhTPiDOtmCUry77fOffIp6peCv5BcNF-_MGV7upON-Itl-84F52q20fVSnRa1nqt1eNq9Q-5qJ6l9JNzrkXDn1YXQspGFs2q-rPdI0s2IgYKt2zyDH_nCDYn5uM0susp0JT3MMA4pwJOMaPdUyB6zzYRBnI0j-xAIUAmT65cIDi2CS5OtxEOe0rsAIHsPEAG5qdY5pnqsThGgoGVVXRH-cjmtATIJc5Q3iAjc7g_LjYYICGDlOD4vHriYUj44nxeVtsvn7dXX-ub79ffrjY3tVW9yLXXrdRCePDY6045bFrnZec1bzvvG22lRdl3jrdW7YRUzjWub9a91bsWQKjL6s3J9hCnXzOmbEZKFocBAk5zMlqKUoJqC7g-gTZOKUX05hBphHg0gpulJ3Pfk1lKMFya-57Mont1XjDvRnQPqnMxBXh9BiBZGHyEYCk9cKrnrVK8cJ9OHJbfuCOMJlnCYNFRRJuNm-g_Uf4Crv60iQ</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Siti Najila, M.J</creator><creator>Noor Rain, A</creator><creator>Mohamad Kamel, A.G</creator><creator>Syed Zahir, S.I</creator><creator>Khozirah, S</creator><creator>Lokman Hakim, S</creator><creator>Zakiah, I</creator><creator>Azizol, A.K</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay</title><author>Siti Najila, M.J ; Noor Rain, A ; Mohamad Kamel, A.G ; Syed Zahir, S.I ; Khozirah, S ; Lokman Hakim, S ; Zakiah, I ; Azizol, A.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-f762711fafe9783de46df28f7068ff47c2ce298d06c3b123dd4d9459c7b6aa13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aldehyde Oxidoreductases - metabolism</topic><topic>Andrographis</topic><topic>Animals</topic><topic>Annonaceae</topic><topic>Antimalarials - isolation & purification</topic><topic>Antimalarials - pharmacology</topic><topic>Antimalarials - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - methods</topic><topic>Biological Assay - statistics & numerical data</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>In vitro anti-malarial activity</topic><topic>Lactate dehydrogenase assay</topic><topic>Medical sciences</topic><topic>Medicinal plants</topic><topic>Pharmacognosy. 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Drug treatments</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - therapeutic use</topic><topic>Plant Leaves</topic><topic>Plant Stems</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siti Najila, M.J</creatorcontrib><creatorcontrib>Noor Rain, A</creatorcontrib><creatorcontrib>Mohamad Kamel, A.G</creatorcontrib><creatorcontrib>Syed Zahir, S.I</creatorcontrib><creatorcontrib>Khozirah, S</creatorcontrib><creatorcontrib>Lokman Hakim, S</creatorcontrib><creatorcontrib>Zakiah, I</creatorcontrib><creatorcontrib>Azizol, A.K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siti Najila, M.J</au><au>Noor Rain, A</au><au>Mohamad Kamel, A.G</au><au>Syed Zahir, S.I</au><au>Khozirah, S</au><au>Lokman Hakim, S</au><au>Zakiah, I</au><au>Azizol, A.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>82</volume><issue>2</issue><spage>239</spage><epage>242</epage><pages>239-242</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><coden>JOETD7</coden><abstract>Goniothalamus scortechinii,
Andrographis paniculata and
Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards
Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of
G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of
Plasmodium falciparum. Furthermore a better IC
50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC
50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>12242001</pmid><doi>10.1016/S0378-8741(02)00183-6</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0378-8741 |
| ispartof | Journal of ethnopharmacology, 2002-10, Vol.82 (2), p.239-242 |
| issn | 0378-8741 1872-7573 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Aldehyde Oxidoreductases - metabolism Andrographis Animals Annonaceae Antimalarials - isolation & purification Antimalarials - pharmacology Antimalarials - therapeutic use Biological and medical sciences Biological Assay - methods Biological Assay - statistics & numerical data Drug Evaluation, Preclinical - methods General pharmacology Humans In vitro anti-malarial activity Lactate dehydrogenase assay Medical sciences Medicinal plants Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Extracts - therapeutic use Plant Leaves Plant Stems Plasmodium falciparum Plasmodium falciparum - drug effects |
| title | The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay |
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