The influence of parathyroid hormone-related protein (PTHrP) on tooth-germ development and osteoclastogenesis in alveolar bone of PTHrP-knock out and wild-type mice in vitro

In a previous study, it was shown that tooth germs of neonatal homozygous parathyroid hormone-related protein (PTHrP)-knockout mice are penetrated or compressed by the surrounding alveolar bone, suggesting an important role for PTHrP in the formation and activation of osteoclasts around growing toot...

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Bibliographic Details
Published in:Archives of oral biology 2002-09, Vol.47 (9), p.665-672
Main Authors: Mekaapiruk, Kongkwan, Suda, Naoto, Hammond, Vicki E, Beck, Felix, Kuroda, Takayuki, Takano, Yoshiro, Terashima, Tatsuo
Format: Article
Language:English
Subjects:
Acid Phosphatase - analysis
Alveolar bone
Alveolar Process - embryology
Animals
Cell Division - physiology
Dentistry
Female
Gene-deficient mouse
Isoenzymes - analysis
Male
Mice
Mice, Knockout
Organ culture
Organ Culture Techniques
Osteoclasts
Osteoclasts - cytology
Parathyroid Hormone-Related Protein
Peptide Hormones - genetics
Peptide Hormones - physiology
PTHrP
Tartrate-Resistant Acid Phosphatase
Tooth germ
Tooth Germ - physiology
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Summary:In a previous study, it was shown that tooth germs of neonatal homozygous parathyroid hormone-related protein (PTHrP)-knockout mice are penetrated or compressed by the surrounding alveolar bone, suggesting an important role for PTHrP in the formation and activation of osteoclasts around growing tooth germs. In order to elucidate the role of PTHrP during the development of the tooth germ and related structures, mandibular explants containing cap stage tooth germs of embryonic day 14, homozygous mice were here cultured with or without surrounding alveolar bone. There was no difference in the number of tartrate-resistant acid phosphatase-positive multinucleated osteoclastic cells around the first molars of homozygous and wild-type mice. After 10 days of culture, osteoclastic cells were rarely present in explants from homozygous mice and penetration of alveolar bone into the dental papilla was observed. The decline in osteoclast number was partly restored by the addition of PTHrP to the culture. Tooth germs of both wild-type and homozygous mice cultured without alveolar bone developed well, with no apparent structural abnormality; dentine formation was evident after 10 days. These data suggest that PTHrP is not required for the development of the tooth germ proper but is indispensable in promoting the osteoclast formation required to accommodate that development.
ISSN:0003-9969
1879-1506
DOI:10.1016/S0003-9969(02)00026-2