Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique

Fluorine-18 fluoro-2-deoxyglucose ((18)FDG) and carbon-14 2-deoxyglucose ((14)C-2-DG) are both widely used tracers of myocardial glucose uptake and phosphorylation. We have recently shown, using positron emission tomography (PET) and nuclear magnetic resonance, that ischaemia-reperfusion (I-R) cause...

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Bibliographic Details
Published in:European journal of nuclear medicine and molecular imaging 2002-10, Vol.29 (10), p.1334-1341
Main Authors: SOUTHWORTH, Richard, DEARLING, Jason L. J, MEDINA, Rodolfo A, FLYNN, Aiden A, PEDLEY, R. Barbara, GARLICK, Pamela B
Format: Article
Language:English
Subjects:
Animals
Autoradiography - methods
Biological and medical sciences
Carbon Radioisotopes - pharmacokinetics
Cardiovascular system
Cerebrovascular Circulation
Deoxyglucose - pharmacokinetics
Fluorodeoxyglucose F18 - pharmacokinetics
Glucose - metabolism
Glucose Transporter Type 1
Glucose Transporter Type 2
Heart Ventricles - metabolism
Heart Ventricles - pathology
In Vitro Techniques
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Monosaccharide Transport Proteins - metabolism
Myocardial Ischemia - metabolism
Myocardial Ischemia - pathology
Myocardial Reperfusion
Myocardium - metabolism
Quality Control
Radionuclide investigations
Radiopharmaceuticals - pharmacokinetics
Rats
Rats, Wistar
Reference Values
Reproducibility of Results
Sensitivity and Specificity
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Summary:Fluorine-18 fluoro-2-deoxyglucose ((18)FDG) and carbon-14 2-deoxyglucose ((14)C-2-DG) are both widely used tracers of myocardial glucose uptake and phosphorylation. We have recently shown, using positron emission tomography (PET) and nuclear magnetic resonance, that ischaemia-reperfusion (I-R) causes differential changes in their uptake. We describe here the novel application of an autoradiographic technique allowing the investigation of this phenomenon at high resolution, using tracer concentrations of both analogues in the dual-perfused isolated rat heart. We also investigate the importance of glucose transporter (GLUT 1 and GLUT 4) distribution in governing the observed phosphorylated analogue accumulation. Hearts ( n=5) were perfused with Krebs buffer for 40 min, made regionally zero-flow ischaemic for 40 min and reperfused for 60 min with Krebs containing tracer (18)FDG (200 MBq) and tracer (14)C-2-DG (0.37 MBq). Hearts were then frozen and five sections (10 micro m) were cut per heart, fixed and exposed on phosphor storage plates for 18 h (for (18)FDG) and then for a further 9 days (for (14)C-2-DG). Quantitative digital images of tracer accumulation were obtained using a phosphor plate reader. The protocol was repeated in a second group of hearts and GLUT 1 and GLUT 4 distribution analysed. Post-ischaemic accumulation of (18)FDG-6-P was inhibited by 38.2%+/-1.7% and (14)C-DG-6-P by 19.0%+/-2.2%, compared with control ( P
ISSN:1619-7070
1619-7089
DOI:10.1007/s00259-002-0922-2