Libraries of Composite Polyfluors Built from Fluorescent Deoxyribosides

We report on a new class of water-soluble fluorescent molecules (polyfluors) that are composed of multiple individual fluorophores assembled on a DNA-like backbone. Four fluorophore deoxyribosides were synthesized, and these individual molecules were assembled into oligofluor strings on a DNA synthe...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2002-10, Vol.124 (39), p.11590-11591
Main Authors: Gao, Jianmin, Strässler, Christoph, Tahmassebi, Deborah, Kool, Eric T
Format: Article
Language:English
Subjects:
Deoxyribonucleosides - chemistry
DNA, Single-Stranded - chemistry
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Pyrenes - chemistry
Spectrometry, Fluorescence
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Summary:We report on a new class of water-soluble fluorescent molecules (polyfluors) that are composed of multiple individual fluorophores assembled on a DNA-like backbone. Four fluorophore deoxyribosides were synthesized, and these individual molecules were assembled into oligofluor strings on a DNA synthesizer. A library of 256 tetrafluors was generated by split and pool methods on polystyrene beads. Images of the library under a fluorescence microscope revealed at least 40−50 different hues and intensities. Selected tetrafluors were resynthesized in pure form in solution and displayed properties, such as large Stokes shifts, that individual fluorophores do not have.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja027197a