Cloning and expression of a novel retinoblastoma binding protein cDNA, RBBP10

A 2860-bp cDNA was isolated from a human fetal brain cDNA library by high throughput cDNA sequencing, which encodes a putative protein with 186 amino acids. The putative protein shares 90.7% identity with rat pBOG (3403163) and shares 93.4% identity with human RBBP9 (NP_006597.1). A conserved RB bin...

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Bibliographic Details
Published in:Biochemical genetics 2002-08, Vol.40 (7-8), p.273-282
Main Authors: Chen, Jin-Zhong, Yang, Quan-Sheng, Wang, Shu, Meng, Xian-Fang, Ying, Kang, Xie, Yi, Ma, Yu-Min
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Brain
Brain - metabolism
Carrier Proteins - genetics
Cell Cycle Proteins
Cell Line
chromosome 20
Chromosome Mapping
Chromosomes, Human, Pair 20
Cloning
Cloning, Molecular
COS Cells
Cytoplasm
DNA, Complementary - analysis
Exons
Fetuses
Gene Expression
Gene Library
Genomes
Humans
Intracellular Signaling Peptides and Proteins
Introns
Molecular Sequence Data
Mutation
Neoplasm Proteins
Nerve Tissue Proteins - genetics
Open Reading Frames
Polymerase chain reaction
retinoblastoma
Retinoblastoma - genetics
Retinoblastoma protein
Reverse Transcriptase Polymerase Chain Reaction
Tissue Distribution
Transfection
Tumors
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Description
Summary:A 2860-bp cDNA was isolated from a human fetal brain cDNA library by high throughput cDNA sequencing, which encodes a putative protein with 186 amino acids. The putative protein shares 90.7% identity with rat pBOG (3403163) and shares 93.4% identity with human RBBP9 (NP_006597.1). A conserved RB binding domain, L x C x E, located between residue 63 and 68 was recognized. Therefore, it was named RBBP10. Mapviewer analysis locates it on human chromosome 20q11.22. RBBP10 spans about 9.6 kb of the genome and consists of six exons and five introns. RT-PCR revealed that the gene was expressed widely in various human tissues, and the expression level is somewhat higher in tumor tissues than in normal tissues. But subsequent sequencing analysis did notfound any mutation of this in tumor tissues. The COS 7 cell transfected with the ORF of RBBP10 showed that the protein was distributed both in the cytoplasm and in the nucleus. Our results suggest that RBBP10 is the orthologue of the rat BOG gene (AF025819) and a paralogue of human RBBP9 (AF039564).
ISSN:0006-2928
1573-4927
DOI:10.1023/A:1019886918029