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Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis
The mannose-binding lectin (MBL) (also known as the mannose-binding protein) is a serum protein that plays a role as an “ante-antibody” in innate immunity. In man, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, MBL-A and MBL-C. In order to evaluate the relative r...
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| Published in: | Microbes and infection 2002-07, Vol.4 (8), p.773-784 |
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| container_end_page | 784 |
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| container_title | Microbes and infection |
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| creator | Takahashi, Kazue Gordon, Judith Liu, Hong Sastry, Kedarnath N Epstein, Judy E Motwani, Monica Laursen, Inga Thiel, Steffen Jensenius, Jens Christian Carroll, Michael Ezekowitz, R.Alan B |
| description | The mannose-binding lectin (MBL) (also known as the mannose-binding protein) is a serum protein that plays a role as an “ante-antibody” in innate immunity. In man, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, MBL-A and MBL-C. In order to evaluate the relative roles of these two forms of MBL, we created MBL-A null mice that were MBL-C sufficient. We found MBL-A null mice had enhanced survival in a septic peritonitis model compared to wild-type mice and complement 3 null mice at 24 h, 48 h and 10 d (
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| doi_str_mv | 10.1016/S1286-4579(02)01597-6 |
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P < 0.05). Reconstitution of these mice with human MBL reversed the phenotype. Surviving mice had significantly decreased TNF-α and IL-6 levels in the blood and peritoneal cavity (
P < 0.01). In vitro studies indicate that bacteria opsonized with MBL-A-deficient serum induced significantly less cytokine by peritoneal macrophages compared to those with wild-type serum. Our results indicate that MBL-A is a modulator of inflammation in vivo and in vitro in the mouse and that the role of MBL may extend beyond its role as an opsonin.</description><identifier>ISSN: 1286-4579</identifier><identifier>EISSN: 1769-714X</identifier><identifier>DOI: 10.1016/S1286-4579(02)01597-6</identifier><identifier>PMID: 12270724</identifier><language>eng</language><publisher>Lausanne: Elsevier SAS</publisher><subject>Animals ; Biological and medical sciences ; CLP ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; IL-6 ; Inflammation ; Inflammation - genetics ; Inflammation - immunology ; Interleukin-6 - analysis ; Knockout ; Leukocytes - immunology ; Mannose-binding lectin (MBL) ; Mannose-Binding Lectin - analogs & derivatives ; Mannose-Binding Lectin - deficiency ; Mannose-Binding Lectin - genetics ; Mannose-Binding Lectin - immunology ; Mice ; Mice, Knockout ; Molecular and cellular biology ; Peritonitis - genetics ; Peritonitis - immunology ; PMN ; Septic shock ; Stem Cells ; Survival Analysis ; TNF-α ; Tumor Necrosis Factor-alpha - analysis</subject><ispartof>Microbes and infection, 2002-07, Vol.4 (8), p.773-784</ispartof><rights>2002 Éditions scientifiques et médicales Elsevier SAS</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-9f80b31f88134517d3b93cc86a14c6b67f3a8b163eee97c122cb774e4e273f5f3</citedby><cites>FETCH-LOGICAL-c490t-9f80b31f88134517d3b93cc86a14c6b67f3a8b163eee97c122cb774e4e273f5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13766911$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12270724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takahashi, Kazue</creatorcontrib><creatorcontrib>Gordon, Judith</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Sastry, Kedarnath N</creatorcontrib><creatorcontrib>Epstein, Judy E</creatorcontrib><creatorcontrib>Motwani, Monica</creatorcontrib><creatorcontrib>Laursen, Inga</creatorcontrib><creatorcontrib>Thiel, Steffen</creatorcontrib><creatorcontrib>Jensenius, Jens Christian</creatorcontrib><creatorcontrib>Carroll, Michael</creatorcontrib><creatorcontrib>Ezekowitz, R.Alan B</creatorcontrib><title>Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis</title><title>Microbes and infection</title><addtitle>Microbes Infect</addtitle><description>The mannose-binding lectin (MBL) (also known as the mannose-binding protein) is a serum protein that plays a role as an “ante-antibody” in innate immunity. In man, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, MBL-A and MBL-C. In order to evaluate the relative roles of these two forms of MBL, we created MBL-A null mice that were MBL-C sufficient. We found MBL-A null mice had enhanced survival in a septic peritonitis model compared to wild-type mice and complement 3 null mice at 24 h, 48 h and 10 d (
P < 0.05). Reconstitution of these mice with human MBL reversed the phenotype. Surviving mice had significantly decreased TNF-α and IL-6 levels in the blood and peritoneal cavity (
P < 0.01). In vitro studies indicate that bacteria opsonized with MBL-A-deficient serum induced significantly less cytokine by peritoneal macrophages compared to those with wild-type serum. Our results indicate that MBL-A is a modulator of inflammation in vivo and in vitro in the mouse and that the role of MBL may extend beyond its role as an opsonin.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CLP</subject><subject>Disease Models, Animal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>IL-6</subject><subject>Inflammation</subject><subject>Inflammation - genetics</subject><subject>Inflammation - immunology</subject><subject>Interleukin-6 - analysis</subject><subject>Knockout</subject><subject>Leukocytes - immunology</subject><subject>Mannose-binding lectin (MBL)</subject><subject>Mannose-Binding Lectin - analogs & derivatives</subject><subject>Mannose-Binding Lectin - deficiency</subject><subject>Mannose-Binding Lectin - genetics</subject><subject>Mannose-Binding Lectin - immunology</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Molecular and cellular biology</subject><subject>Peritonitis - genetics</subject><subject>Peritonitis - immunology</subject><subject>PMN</subject><subject>Septic shock</subject><subject>Stem Cells</subject><subject>Survival Analysis</subject><subject>TNF-α</subject><subject>Tumor Necrosis Factor-alpha - analysis</subject><issn>1286-4579</issn><issn>1769-714X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAUhS0Eog_4CSBvQGWR4ms7drxCVVUe0kgsChI7y3GuwZA4g52M1H-PpzOoy258vfjOvUfnEPIK2CUwUO9vgXeqka02F4y_Y9Aa3agn5BS0Mo0G-eNp_f9HTshZKb9ZpbSSz8kJcK6Z5vKU2I3zf-gc6ORSmgs2fUxDTD_piH6JqbmimH655LHQsuZd3LmRxkQdnea1YH0HHPdy59cFacHtEj3dYo7LnOISywvyLLix4MvjPCffP958u_7cbL5--nJ9tWm8NGxpTOhYLyB0HQjZgh5Eb4T3nXIgveqVDsJ1PSiBiEb76t_3WkuUyLUIbRDn5O1h7zbPf1csi51i8TiOLmF1ajUHLrmSj4LQKcYFmAq2B9DnuZSMwW5znFy-s8DsvgJ7X4Hd52sZt_cVWFV1r48H1n7C4UF1zLwCb46AK96NIdd4Y3nghFbKAFTuw4HDmtsuYrbFR6xVDDHXcuwwx0es_APuhqLb</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Takahashi, Kazue</creator><creator>Gordon, Judith</creator><creator>Liu, Hong</creator><creator>Sastry, Kedarnath N</creator><creator>Epstein, Judy E</creator><creator>Motwani, Monica</creator><creator>Laursen, Inga</creator><creator>Thiel, Steffen</creator><creator>Jensenius, Jens Christian</creator><creator>Carroll, Michael</creator><creator>Ezekowitz, R.Alan B</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis</title><author>Takahashi, Kazue ; Gordon, Judith ; Liu, Hong ; Sastry, Kedarnath N ; Epstein, Judy E ; Motwani, Monica ; Laursen, Inga ; Thiel, Steffen ; Jensenius, Jens Christian ; Carroll, Michael ; Ezekowitz, R.Alan B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-9f80b31f88134517d3b93cc86a14c6b67f3a8b163eee97c122cb774e4e273f5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CLP</topic><topic>Disease Models, Animal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>IL-6</topic><topic>Inflammation</topic><topic>Inflammation - genetics</topic><topic>Inflammation - immunology</topic><topic>Interleukin-6 - analysis</topic><topic>Knockout</topic><topic>Leukocytes - immunology</topic><topic>Mannose-binding lectin (MBL)</topic><topic>Mannose-Binding Lectin - analogs & derivatives</topic><topic>Mannose-Binding Lectin - deficiency</topic><topic>Mannose-Binding Lectin - genetics</topic><topic>Mannose-Binding Lectin - immunology</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Molecular and cellular biology</topic><topic>Peritonitis - genetics</topic><topic>Peritonitis - immunology</topic><topic>PMN</topic><topic>Septic shock</topic><topic>Stem Cells</topic><topic>Survival Analysis</topic><topic>TNF-α</topic><topic>Tumor Necrosis Factor-alpha - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takahashi, Kazue</creatorcontrib><creatorcontrib>Gordon, Judith</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Sastry, Kedarnath N</creatorcontrib><creatorcontrib>Epstein, Judy E</creatorcontrib><creatorcontrib>Motwani, Monica</creatorcontrib><creatorcontrib>Laursen, Inga</creatorcontrib><creatorcontrib>Thiel, Steffen</creatorcontrib><creatorcontrib>Jensenius, Jens Christian</creatorcontrib><creatorcontrib>Carroll, Michael</creatorcontrib><creatorcontrib>Ezekowitz, R.Alan B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbes and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takahashi, Kazue</au><au>Gordon, Judith</au><au>Liu, Hong</au><au>Sastry, Kedarnath N</au><au>Epstein, Judy E</au><au>Motwani, Monica</au><au>Laursen, Inga</au><au>Thiel, Steffen</au><au>Jensenius, Jens Christian</au><au>Carroll, Michael</au><au>Ezekowitz, R.Alan B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis</atitle><jtitle>Microbes and infection</jtitle><addtitle>Microbes Infect</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>4</volume><issue>8</issue><spage>773</spage><epage>784</epage><pages>773-784</pages><issn>1286-4579</issn><eissn>1769-714X</eissn><abstract>The mannose-binding lectin (MBL) (also known as the mannose-binding protein) is a serum protein that plays a role as an “ante-antibody” in innate immunity. In man, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, MBL-A and MBL-C. In order to evaluate the relative roles of these two forms of MBL, we created MBL-A null mice that were MBL-C sufficient. We found MBL-A null mice had enhanced survival in a septic peritonitis model compared to wild-type mice and complement 3 null mice at 24 h, 48 h and 10 d (
P < 0.05). Reconstitution of these mice with human MBL reversed the phenotype. Surviving mice had significantly decreased TNF-α and IL-6 levels in the blood and peritoneal cavity (
P < 0.01). In vitro studies indicate that bacteria opsonized with MBL-A-deficient serum induced significantly less cytokine by peritoneal macrophages compared to those with wild-type serum. Our results indicate that MBL-A is a modulator of inflammation in vivo and in vitro in the mouse and that the role of MBL may extend beyond its role as an opsonin.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>12270724</pmid><doi>10.1016/S1286-4579(02)01597-6</doi><tpages>12</tpages></addata></record> |
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| ispartof | Microbes and infection, 2002-07, Vol.4 (8), p.773-784 |
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| source | ScienceDirect Journals |
| subjects | Animals Biological and medical sciences CLP Disease Models, Animal Fundamental and applied biological sciences. Psychology Gene Deletion IL-6 Inflammation Inflammation - genetics Inflammation - immunology Interleukin-6 - analysis Knockout Leukocytes - immunology Mannose-binding lectin (MBL) Mannose-Binding Lectin - analogs & derivatives Mannose-Binding Lectin - deficiency Mannose-Binding Lectin - genetics Mannose-Binding Lectin - immunology Mice Mice, Knockout Molecular and cellular biology Peritonitis - genetics Peritonitis - immunology PMN Septic shock Stem Cells Survival Analysis TNF-α Tumor Necrosis Factor-alpha - analysis |
| title | Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis |
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